10-FnIII scaffold protein anti-Human Albumin (CAT#: SFN-L021)
The tenth fibronectin type III domain (10Fn3) that bound albumin was isolated from the library using phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=5 μΜ) for albumin as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/763_G10_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/763_G10_1_selected.jpg
Figure 2 IHC staining of human fallopian tube
(Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6262/17728_A_9_7_selected.jpg
(Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6262/17728_A_9_7_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_3.jpg
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_5.jpg
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_6_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_6_6.jpg
Figure 7 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163631-ALB
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163631-ALB
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 Loops
- Target
- Albumin
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 5 μΜ
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
- Gene ID
- 213
- UniProt ID
- P02768
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-81-sdAb | Recombinant Anti-human ALB VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| PNBL-002 | Recombinant Anti-Human, mouse, and rat ALB IgNAR Antibody (E06) | ELISA | Shark IgNAR |
| PABX-001-VHH | Recombinant Anti-Human Albumins VHH Single Domain Antibody (E06) | ELISA | Shark VHH |
| NBD-0046-YJ-VHH | Camelid Anti-ALB Recombinant Single Domain Antibody (Nb.b201) | ELISA, FC | Camelid VHH |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0511CL | Anti-Human ALB Recombinant Antibody (dAbL1) | ELISA | Humanized Antibody |
| TAB-0512CL | Anti-Human ALB Recombinant Antibody (dAbH1) | ELISA | Humanized Antibody |
| TAB-0513CL | Anti-Human ALB Recombinant Antibody (dAbL2) | ELISA | Humanized Antibody |
| TAB-0514CL | Anti-Human ALB Recombinant Antibody (JAbH2) | ELISA | Humanized Antibody |
| TAB-0515CL | Anti-Human ALB Recombinant Antibody (FabA-didAb) | ELISA | Humanized Antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-431CT | Anti-Human Serum Albumin Recombinant Antibody (DOM7r31) | WB, ELISA | Human antibody |
| TAB-432CT | Anti-Human Serum Albumin Recombinant Antibody (DOM7r36) | WB, ELISA | Human antibody |
| TAB-433CT | Anti-Human Serum Albumin Recombinant Antibody (DOM7r92) | WB, ELISA | Human antibody |
| TAB-431CT-F(E) | Anti-Human Serum Albumin Recombinant Antibody Fab Fragment (DOM7r31) | WB, ELISA | Human antibody |
| TAB-432CT-F(E) | Anti-Human Serum Albumin Recombinant Antibody Fab Fragment (DOM7r36) | WB, ELISA | Human antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0579MZ | Mouse Anti-ALB Recombinant Antibody (clone LU43) | ELISA, WB | Mouse IgG1 |
| MRO-0047-CN | Rabbit Anti-ALB Recombinant Antibody (clone JF32-10) | WB, IHC | Rabbit IgG |
| MRO-1633-CN | Rabbit Anti-ALB Polyclonal Antibody (MRO-1633-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0663-WJ | Human Anti-ALB Recombinant Antibody (HPAB-0663-WJ) | ELISA | Humanized IgG |
| PABS-0046 | Rabbit Anti-ALB Recombinant Antibody (PABS-0046) | ELISA, FuncS | Rabbit IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0672MZ | Chicken Anti-Albumin Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0132 | Hi-Affi™ Rabbit Anti-ALB Recombinant Antibody (clone DS132AB) | WB, IHC, FC | Rabbit IgG |
| MOR-0133 | Hi-Affi™ Rabbit Anti-ALB Recombinant Antibody (clone DS133AB) | ELISA, WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0663-WJ-F(E) | Human Anti-ALB Recombinant Antibody; Fab Fragment (HPAB-0663-WJ-F(E)) | ELISA | Humanized Fab |
| PFBS-0046 | Rabbit Anti-ALB Recombinant Antibody; Fab Fragment (PFBS-0046) | ELISA, FuncS | Rabbit Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0663-WJ-S(P) | Human Anti-ALB Recombinant Antibody; scFv Fragment (HPAB-0663-WJ-S(P)) | ELISA | Human scFv |
| PSBS-0046 | Rabbit Anti-ALB Recombinant Antibody; scFv Fragment (PSBS-0046) | ELISA, FuncS | Rabbit scFv |
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