10-FnIII scaffold protein anti-Chicken Lysozyme (CAT#: SFN-L013)
The tenth fibronectin type III domain (10Fn3) that bound lysozyme was isolated from the library using mRNA Display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=0.35 nM) for lysozyme as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
Figure 2 IHC staining of human salivary gland
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
Figure 3 IHC staining of human skeletal muscle
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
Figure 4 Colon
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- Lysozyme
- Species Reactivity
- Chicken
- Expression Host
- E. coli
- Affinity Constant
- 0.35 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3387z | Mouse Anti-LYZ Recombinant Antibody (clone 39H8) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
| PABZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5) | WB, FuncS | Mouse IgG |
| PABZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1) | WB, IF, FuncS | Mouse IgG |
| PABW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4) | ELISA | Human IgG |
| PABC-023 | Recombinant Mouse Anti-Chicken LYZ Antibody (F10.6.6) | WB, IF, FuncS | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10); scFv Fragment | WB, IHC, FuncS | Human scFv |
| PSBL-426 | Human Anti-LYZ Recombinant Antibody; scFv Fragment | WB, IF, FuncS | Human scFv |
| MOB-111YJL-S(P) | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D); scFv Fragment | ELISA, FC, IHC | Human scFv |
| MOB-112YJL-S(P) | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D,Y58F); scFv Fragment | ELISA, FC, IHC | Human scFv |
| PSBS-0138 | Human Anti-LYZ Recombinant Antibody (Clone 01JF38); scFv Fragment | ELISA, FuncS | Human scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-023 | Recombinant Anti-Human LYZ VHH Single Domain Antibody (cAbHuL5) | WB, ELISA, FuncS | Llama VHH |
| PNBL-024 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody () | WB, ELISA, FuncS | Llama VHH |
| PNBL-036 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (1D2L19) | WB, FuncS | Llama VHH |
| PNBL-051 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAbBCII-10) | ELISA | Llama VHH |
| PNBL-052 | Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (cAb-HuL6) | WB, ELISA, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); Fab Fragment | WB, FuncS | Mouse Fab |
| FAMAB-0212CQ-F(E) | Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Hyb.C1) | ELISA | Mouse Fab |
| FAMAB-0213CQ-F(E) | Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Gloop2) | ELISA | Mouse Fab |
| FAMAB-0214CQ-F(E) | Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Gloop3) | ELISA | Mouse Fab |
| FAMAB-0215CQ-F(E) | Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Gloop5) | ELISA | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
| PABX-073-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) | WB, ELISA, FuncS | Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-074 | Recombinant Mouse Anti-Lysozyme Antibody (HyHEL-5) | WB, ELISA, FuncS | IgG |
| PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
| PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
| PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) | WB, ELISA, FuncS | scFv |
| PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5) | WB, ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
| PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
| PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
MHC Tetramer for Other Applications
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2112 | Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) | ICC, IHC-P, IP, WB | IgG |
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