10-FnIII scaffold protein anti-Human The Src-homology 2 (SH2) domain-containing phosphatase 2 (SHP2) (CAT#: SFN-L025)
The tenth fibronectin type III domain (10Fn3) that bound the Src-homology 2 (SH2) domain-containing phosphatase 2 (SHP2) was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities for the Src-homology 2 (SH2) domain-containing phosphatase 2 (SHP2) as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
(Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
Figure 4 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 Loops
- Target
- The Src-homology 2 (SH2) domain-containing phosphatase 2 (SHP2)
- Species Reactivity
- Human
- Expression Host
- E. coli
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
- Gene ID
- 5728
- UniProt ID
- P60484
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