CBGlyco™ 6xHis Protein Tag Stain Kit (CAT#: Glyco-048CL)
6xHis Protein Tag Stain Reagent Set is a fluorescent stain for preferential detection of histidine-tagged (His-tagged) recombinant fusion proteins in protein polyacrylamide gels.
Specific Inquiry
Specifications
- Product Size
- 1 L
- Target Molecule
- Proteins (His-Tagged)
- Label or Dye
- Proprietary Mix
- Detection Method
- Fluorescent
- Detection Location
- In-Gel Detection
- Contents & storage
- Store components at room temperature. Product is shipped at ambient temperature
Background
- Description
- The unique fluorescent stain specifically detects histidine-tagged fusion proteins that have been electrophoresed in polyacrylamide gels (e.g., SDS-PAGE). With this stain, expression of His-tagged recombinant proteins can be assessed directly in-gel, eliminating membrane transfer and Western blotting steps that are typically used to confirm purity and yield of tagged proteins. Although the lower limit of detection depends on which UV lamp (300nm) and detection equipment (CCD camera best) are used, the stain is convenient for in-process assessment of His-tagged protein production and is compatible with subsequent staining with coomassie dye and other total protein stains.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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