Products

CBGlyco™ Aminolink Coupling Resin (Glyco-031CL)

Aminolink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.      

Specifications
Product Size
10 mL
50 mL
Format
Bottle(s)
Column Type
Affinity, Agarose Resin
Immobilized Phase
Aldehyde
Contents & storage
Store at 4°C.
BACKGROUND
Description
Proteins and other molecules with primary amines can be covalently attached (immobilized) to Aminolink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

The Aminolink Resin immobilization reaction involves spontaneous formation of Schiff base bonds between aldehydes and amines and their subsequent stabilization by incubation with a mild reductant. The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20 mg of immobilized protein per milliliter of resin.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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