Ankyrin repeat scaffold protein anti-Human IL4 (CAT#: SAR-L261)
The Ankyrin repeat that bound IL4 was isolated from the library using ribosome display techonology. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops, first discovered in signaling proteins in yeast Cdc10 and Drosophila Notch. Domains consisting of ankyrin repeats mediate protein–protein interactions and are among the most common structural motifs in known proteins. After affinity maturation, Ankyrin repeat variants were identified having affinities (KD=9.0 pM) for IL4 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg
Figure 2 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113520-IL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113520-IL4
Specifications
- Scaffold Name
- Ankyrin repeat
- Origin
- Ankyrin repeat proteins
- Core Structure
- 2α-helices, β-turn
- Variable Regions
- b-turn,1a-helix and loop
- Target
- IL4
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 9.0 pM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
Related Resources
Asthma
Autoimmune Thyroid Disease
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Il4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-102 | Mouse Anti-Il4 Recombinant Antibody (clone 11B11) | ELISA, FC | Mouse IgG |
| MOB-0156MZ | Recombinant Mouse Anti-IL-4 Antibody (clone A12-61) | ELISA | Mouse antibody |
| FN-084CQ | Rat Anti-Il4 Recombinant Antibody (clone BVD6-24G2) | FC | Rat IgG1 |
| FN-125CQ | Mouse Anti-IL4 Recombinant Antibody (clone CBL-1305) | FuncS, Neut, Inhib | Mouse IgG1 |
| ZG-0746F | Rat Anti-IL4 Antibody (clone AR.98) | FC, IF | Rat IgG1 |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-221 | Anti-Human IL4 Recombinant Antibody (Pascolizumab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - kappa |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-666LC | Anti-human IL4 Recombinant Antibody (6A1) (TAB-666LC) | ELISA, FC | |
| TAB-666LC-S(P) | Anti-human IL4 scFv Fragment (6A1) | ELISA, FC | |
| TAB-666LC-F(E) | Anti-human IL4 Fab Fragment (6A1) | ELISA, FC | |
| MOB-0341MC | Recombinant Mouse Anti-IL4 Antibody | WB, Neut |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-667LC | Human Anti-IL4 Recombinant Antibody (TAB-667LC) | ELISA, FC | Human IgG1 |
| TAB-667LC-S(P) | Human Anti-IL4 Recombinant Antibody; scFv Fragment (TAB-667LC-S(P)) | ELISA, FC | Human scFv |
| TAB-667LC-F(E) | Human Anti-IL4 Recombinant Antibody; Fab Fragment (TAB-667LC-F(E)) | ELISA, FC | Human Fab |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1403CQ | Rat Anti-IL4 Recombinant Antibody (clone 25D2), Biotin | Block, ELISA, IHC, WB | Rat IgG1 |
| NEUT-1420CQ | Rat Anti-Il4 Recombinant Antibody (clone I4.F5) | Block, DB | Rat IgG1 |
| NEUT-1428CQ | Rat Anti-Il4 Recombinant Antibody (clone 1D11) | Block, ELISA, FC, ICC, IF, WB | Rat IgG2b |
| NEUT-1429CQ | Rat Anti-Il4 Recombinant Antibody (clone 24G2) | Block, ELISA, ICC, IF, IHC, WB | Rat IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1423CQ | Rat Anti-Il4 Recombinant Antibody (clone CBL108) | FC, IHC, IP, Neut | Rat IgG1 |
| NEUT-1424CQ | Rat Anti-Il4 Recombinant Antibody (clone CBL238) | ELISA, Neut | Rat IgG2b |
| NEUT-1425CQ | Rat Anti-Il4 Recombinant Antibody (clone CBL899) | ELISA, FC, IF, Neut, WB | Rat IgG2b, κ |
| NEUT-1426CQ | Rat Anti-Il4 Recombinant Antibody (clone CBL993) | Neut, ELISA | Rat IgG1 |
| NEUT-1427CQ | Rat Anti-Il4 Recombinant Antibody (clone 11B11) | ELISA, CyTOF, ELISPOT, Neut, ICC, IP, IHC | Rat IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1812 | Rabbit Anti-IL4 Recombinant Antibody (clone DS1812AB) | ICC, WB | Rabbit IgG |
| MOR-4273 | Rabbit Anti-IL4 Recombinant Antibody (clone SI356DS) | ELISA | Rabbit IgG |
| MOR-4621 | Rabbit Anti-Il4 Recombinant Antibody (clone TH134DS) | WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0079-YC-S(P) | Mouse Anti-IL4 Recombinant Antibody (clone m3B9); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0080-YC-S(P) | Human Anti-IL4 Recombinant Antibody (clone hu3B9); scFv Fragment | ELISA | Humanized scFv |
| HPAB-1537WJ-S(P) | Human Anti-IL4 Recombinant Antibody; scFv Fragment (clone h25D2-9) | ELISA, WB, Neut | Human scFv |
| HPAB-1539WJ-S(P) | Mouse Anti-IL4 Recombinant Antibody; scFv Fragment (clone 3B9) | ELISA, WB, FC | Mouse scFv |
| HPAB-1540WJ-S(P) | Human Anti-IL4 Recombinant Antibody; scFv Fragment (clone ch3B9) | ELISA, WB, FC | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0079-YC-F(E) | Mouse Anti-IL4 Recombinant Antibody (clone m3B9); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0080-YC-F(E) | Human Anti-IL4 Recombinant Antibody (clone hu3B9); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0081-YC-F(E) | Mouse Anti-IL4 Recombinant Antibody (clone m19C11); Fab Fragment | Block, FuncS | Mouse Fab |
| HPAB-0082-YC-F(E) | Human Anti-IL4 Recombinant Antibody (clone hu19C11); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1537WJ-F(E) | Human Anti-IL4 Recombinant Antibody; Fab Fragment (clone h25D2-9) | ELISA, WB, Neut | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-221 | Afuco™ Anti-IL4 ADCC Recombinant Antibody (Pascolizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for SAR-L261. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.