Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (CAT#: HPAB-0273-YC)

The antibodies bind to the second extracellular loop of the human β1-adrenoreceptor (β1-AR-ECII). The antibodies are particularly useful in determination of auto-anti-β1-AR antibodies in an in vitro cell based assay system in order to characterize and to identify auto-antibodies directed against the β1-AR-ECII in a biological sample.


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SDS-PAGE

Figure 1 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SDS-PAGE

Figure 1 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-0273-YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

SEC-HPLC

Figure 2 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SEC-HPLC

Figure 2 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SEC-HPLC

The purity of this antibody is> 95% as determined by SEC-HPLC.

WB

Figure 3 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in WB

Figure 3 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in WB

Western blot analysis of HPAB-0273-YC was performed by loading ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).

HPAB-0273-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in ELISA

Figure 4 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in ELISA

ELISA analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).

The secondary antibody: HRP-Goat anti-Mouse IgG

DB

Figure 5 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in DB

Figure 5 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in DB

Dot Blot analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).

The secondary antibody: HRP-Goat anti-Mouse IgG


Specifications

  • Immunogen
  • GST fusion protein linked with a 31-meric peptide (GST-131-AR-ECII)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human ADRB1
  • Species Reactivity
  • Human
  • Clone
  • DSM ACC3121
  • Applications
  • ELISA
  • Related Disease
  • DCM, ICM

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ADRB1; adrenoceptor beta 1; RHR; B1AR; ADRB1R; BETA1AR; Beta1-adrenoreceptor; Beta1-A

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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