Anti-Human APP Recombinant Antibody (2B3) (CAT#: TAB-0847CLV)

Figure 1 Representative Western blot of MOG-G-UVW lysate (50μg) detected with either 2B3 or 2B12 at 2μg/ml on a 10% gel.

Both antibodies detected full length APP at 103KDa (±2.5 KDa) and a smaller protein at 56KDa (±0.6 KDa), which was most likely a thrombin cleavage fragment of APP.

Thomas, R. S., Liddell, J. E., & Kidd, E. J. (2011). Anti‐amyloid precursor protein immunoglobulins inhibit amyloid‐β production by steric hindrance. The FEBS journal, 278(1), 167-178.

Figure 2 Differential 2B3 and 2B12 recognition of sAPPα and sAPPβ as determined by indirect ELISA.

Data are expressed as a mean (± SEM) % of the standard antibody (6E10) at 0.1μg/ml. 2B12 recognised sAPPα and sAPPβ equally well, however, significantly more 2B3 bound to sAPPα than to sAPPβ.

Thomas, R. S., Liddell, J. E., & Kidd, E. J. (2011). Anti‐amyloid precursor protein immunoglobulins inhibit amyloid‐β production by steric hindrance. The FEBS journal, 278(1), 167-178.