Human Anti-AXL Recombinant Antibody (clone h#11B7-T5) (CAT#: HPAB-0114-LS)

The humanized antibody binds to the extracellular domain of the AXL receptor tyrosine kinase, which at least partially inhibit AXL activity.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 ELISA experiments to investigate the effects of humanized 11B7 anti-AxI antibodies on AxI receptor phosphorylation.

Figure 1 ELISA experiments to investigate the effects of humanized 11B7 anti-AxI antibodies on AxI receptor phosphorylation.

Hs578T breast cancer cells (top) and NCI-H292 lung cancer cells (bottom) were starved, pre-incubated with 10 mg/ml of Gammagard control antibody, the chimeric anti-Axl mAb chm11 B7 (lgG1/Kappa) as well as the humanized anti-AxI antibodies h#11 B7-T2, h#11 B7-T3, h#11B7-T4, h#11B7-T5, and h#11B7-T6 (T2-T6), treated with or without 400 ng/ml mGas6, and lysed. Lysates were transferred to anti-phospho-tyrosine antibody 4G10-coated Maxi-Sorp 96 well plates, which then were washed and incubated with 0.125 mg/ml biotinylated rat anti-Axl antibody 12B7, AP-conjugated streptavidin and AttoPhos substrate solution in order to collect fluorescence intensities. See text for details. Compared with Gammagard control antibody, the chimeric anti-Axl antibody chm11B7 as well as the humanized anti-Axl antibodies h#11B7-T2, h#11B7-T3, h#11 B7-T4, h#11B7-T5, and h#11B7-T6 were able to block or significantly reduce Gasδ-mediated AxI activation in both cell lines as indicated by decreased AxI tyrosine phosphorylation levels in Gasδ-stimulated versus corresponding non-stimulated cells.

ELISA

Figure 2 ELISA experiments to investigate the effects of humanized 11D5 anti-Axl antibodies on AxI receptor phosphorylation.

Figure 2 ELISA experiments to investigate the effects of humanized 11D5 anti-Axl antibodies on AxI receptor phosphorylation.

Hs578T breast cancer cells were starved, pre-incubated with 10 mg/ml of Gammagard control antibody, the chimeric anti-Axl mAb chm11D5 (lgG1/Kappa) as well as the humanized anti-Axl antibodies h#11D5-T2, h#11D5-T3, h#11D5-T4, h#11D5-T5, and h#11D5-T6, treated with or without 400 ng/ml mGasβ, and lysed. Lysates were transferred to anti-phospho-tyrosine antibody 4G10-coated Maxi-Sorp 96 well plates, which then were washed and incubated with 0.125 mg/ml biotinylated rat anti-Axl antibody 12B7, AP-conjugated streptavidin and AttoPhos substrate solution in order to collect fluorescence intensities. See text for details. Compared with Gammagard control antibody, the chimeric anti-Axl antibody chm11D5 as well as the humanized anti-Axl antibodies h#11 D5-T2, h#11 D5-T3, h#11 D5-T4, h#11D5-T5, and h#11D5-T6 were able to block or significantly reduce Gas6-mediated AxI activation as indicated by decreased AxI tyrosine phosphorylation levels in Gasδ-stimulated versus corresponding non-stimulated cells.

ELISA

Figure 3 ELISA experiments to investigate the effects of humanized 11B7 anti-Axl antibodies on AxI receptor phosphorylation.

Figure 3 ELISA experiments to investigate the effects of humanized 11B7 anti-Axl antibodies on AxI receptor phosphorylation.

Hs578T breast cancer cells (top) and NCI-H292 lung cancer cells (bottom) were starved, pre-incubated with no antibody or 10 mg/ml of Gammagard control antibody as well as the humanized anti-Axl antibodies 11 B7-T5, 11B7-T6, 11 B7-T11, 11B7-T23, and 11 B7-T25, treated with or without 400 ng/ml mGasθ, and lysed. Lysates were transferred to anti-phospho-tyrosine antibody 4G10-coated Maxi-Sorp 96 well plates, which then were washed and incubated with 0.125 mg/ml biotinylated rat anti-Axl antibody 12B7, AP-conjugated streptavidin and AttoPhos substrate solution in order to collect fluorescence intensities. See text for details. Compared with Gammagard control antibody, the humanized anti-Axl antibodies 11 B7-T5, 11 B7-T6, 11 B7-T11, 11 B7-T23, and 11B7-T25 significantly reduced Gas6-mediated AxI activation in both cell lines as indicated by decreased AxI tyrosine phosphorylation levels in Gas6-stimulated versus corresponding non-stimulated cells.

FC

Figure 4 FACS analysis to investigate the effects of humanized 11B7 anti-Axl antibodies on AxI receptor internalization.

Figure 4 FACS analysis to investigate the effects of humanized 11B7 anti-Axl antibodies on AxI receptor internalization.

Hs578T breast cancer cells were starved, incubated with no antibody or 10 mg/ml of Gammagard control antibody as well as the humanized anti-Axl antibodies 11B7-T5, 11 B7-T6, 11B7-T11, 11 B7-T23, and 11 B7-T25 for the indicated periods of time, and fixed with formaldehyde. Cells were stained with rat anti-Axl mAb 2A1 primary antibody and PE-conjugated donkey-anti-rat IgG secondary antibody, and subjected to FACS analysis. See text for details. In contrast to Gammagard control antibody, treatment of Hs578 breast cancer cells with the humanized anti-Axl antibodies 11 B7-T5, 11B7-T6, 11B7-T11, 11B7-T23, and 11B7-T25 leads to internalization of the AxI receptor. Relative AxI expression levels in %, defined as mean fluorescence intensity of anti-Axl mAb treated samples relative to untreated samples of the respective treatment period is shown.

FuncS

Figure 5 Spheroid-based cellular angiogenesis assay to investigate the effects of humanized 11B7 anti-Axl antibodies on Gas6-induced endothelial cell sprouting.

Figure 5 Spheroid-based cellular angiogenesis assay to investigate the effects of humanized 11B7 anti-Axl antibodies on Gas6-induced endothelial cell sprouting.

VEGF-A pre-treated HUVEC spheroids were embedded in a 3D collagen gel, stimulated with 1 mg/ml human Gas6 and treated with indicated concentrations of Gammagard control antibody as well as the humanized anti-Axl antibodies 11B7-T5 for 24 h. The mean+SEM of the cumulative sprout length of 10 randomly selected spheroids per data point was analyzed (left panel) and the relative inhibition by the antibody was determined (right panel). Fitting of IC50 curves and calculation of IC50 values was performed with GraphPad Prism 4.03.

ELISA

Figure 6 Binding activity of the rat anti-human AXL humanized antibodies h#11 D5-T1 to h#11 D5-T6 determined by ELISA using human AXL-Fc coated plate.

Figure 6 Binding activity of the rat anti-human AXL humanized antibodies h#11 D5-T1 to h#11 D5-T6 determined by ELISA using human AXL-Fc coated plate.


Specifications

  • Immunogen
  • AXL
  • Host Species
  • Human
  • Type
  • Humanized IgG
  • Specificity
  • Human AXL
  • Species Reactivity
  • Human
  • Clone
  • h#11B7-T5
  • Applications
  • Inhib, ELISA, FC, FuncS
  • Related Disease
  • Squamous cell cancer, Small-cell lung cancer, Gastric cancer, Pancreatic cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Functional Assay.

Target

  • Alternative Names
  • ARK; UFO; JTK11; Tyro7

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone h#11B7-T5"

See other products for "AXL"

Humanized Antibody

Recombinant Antibody

CAT Product Name Application Type
MOB-0872MZ Mouse Anti-AXL Recombinant Antibody (clone 1B3A2) WB Mouse IgG1
HPAB-0600-CN Human Anti-AXL Recombinant Antibody (HPAB-0600-CN) SPR, ELISA, FC, WB, Inhib, FuncS, IHC, Block Human IgG
HPAB-0601-CN Human Anti-AXL Recombinant Antibody (HPAB-0601-CN) ELISA, FC, IHC, Block Human IgG
HPAB-0603-CN Human Anti-AXL Recombinant Antibody (HPAB-0603-CN) WB, ELISA, FC, IHC Chimeric (Mouse/Human) IgG1, κ
HPAB-0604-CN Mouse Anti-AXL Recombinant Antibody (HPAB-0604-CN) ELISA, IHC Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3970 Hi-Affi™ Rabbit Anti-AXL Recombinant Antibody (clone SI30DS) ELISA Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for HPAB-0114-LS. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare