Recombinant Mouse Anti-Aldolase Antibody (93F3) (CAT#: PABX-012)
Anti-Aldolase antibody is a Mouse antibody of IgG1 κ class that binds to an Aldolase. This antibody is a catalytic aldolase antibody. The catalytic efficiency (k cat /K m ) of oneof these aldolase antibodies in theretro-aldol reaction for the best substrate is 4. 5×10 5 s-1 M-1 , which is tenfold greater than that ofthe best characterized aldolase enzyme, 10 fructose-1,6-biphosphate aldolase (4. 9×10 4 s-1 M-1 ).
Specific Inquiry
FuncS
Figure 1 Activation of prodrug 9 and production of drug 2 using aldolase mAb93F3.
Shown are the consumption of 9 and production of 2 over time in the 93F3-catalvzed reaction and the consumption of 9 in the background reaction. In these experiments, compound 9 (500 M) was incubated at 37°C with 93F3 (34 uM) in PBS buffer or PBS buffer alone. The reaction mixtures were periodically analyzed by using LCMS equipped with column, UV detector (254 nm), and EI-MS.
Sinha, S. C., Li, L. S., Watanabe, S. I., Kaltgrad, E., Tanaka, F., Rader, C., ... & Barbas III, C. F. (2004). Aldolase Antibody Activation of Prodrugs of Potent Aldehyde‐Containing Cytotoxics for Selective Chemotherapy. Chemistry–A European Journal, 10(21), 5467-5472.
Inhib
Figure 2 Growth inhibition of human KaposiL's sarcoma (SLK) cell line by prodrug 9 in the presence and absence of 1mm mAb 93F3.
Sinha, S. C., Li, L. S., Watanabe, S. I., Kaltgrad, E., Tanaka, F., Rader, C., ... & Barbas III, C. F. (2004). Aldolase Antibody Activation of Prodrugs of Potent Aldehyde‐Containing Cytotoxics for Selective Chemotherapy. Chemistry–A European Journal, 10(21), 5467-5472.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human Aldolase
- Clone
- 93F3
- Applications
- FuncS, Inhib
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Functional Assay and Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- Aldolase A (ALDOA, or ALDA), also known as fructose-bisphosphate aldolase, is an enzyme that in humans is encoded by the ALDOA gene on chromosome 16. The protein encoded by this gene is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.
- Gene ID
- 101679550
- UniProt ID
- M3YMB2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2521z | Mouse Anti-ALDOA Recombinant Antibody (clone 28D8) | WB, ELISA, FC, ICC, IF | Mouse IgG2a, κ |
| MOB-0572MZ | Mouse Anti-ALDOA Recombinant Antibody (clone 2E6) | IF, WB | Mouse IgG2a |
| MRO-0056-CN | Rabbit Anti-ALDOA Recombinant Antibody (clone JM54-19) | WB, IF, IHC | Rabbit IgG |
| VS3-FY64 | Recombinant Rabbit Anti-ALDOA Antibody (clone R06-6A3) | WB, IHC-F, IHC-P, ICC, IF | Rabbit IgG |
| VS-0923-FY8 | Recombinant Mouse Anti-ALDOA Antibody (VS-0923-FY8) | ELISA, WB | Mouse IgG1 |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-012-S (P) | Recombinant Mouse Anti-Aldolase Antibody scFv Fragment (93F3) | WB, ELISA, FuncS | scFv |
| PABX-012-F (E) | Recombinant Mouse Anti-Aldolase Antibody Fab Fragment (93F3) | WB, ELISA, FuncS | Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0144 | Hi-Affi™ Rabbit Anti-ALDOA Recombinant Antibody (clone DS144AB) | WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1958 | PE-A2/Human ALDOA (ALSDHHIYL) MHC Tetramer | FCM | |
| MHC-LC1984 | PE-A*02:01/Human ALDOA (ALSDHHIYL) MHC Tetramer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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