Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) (CAT#: TAB-1284CL)
This antibody is a monoclonal Antibodies specifically recognizing B-Raf mutant proteins, and can be used to detect whether there are N581S, V600E and V600K mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically.
Specific Inquiry
IB
Figure 1 An immunoblotting assay map of an antibody produced by a cell line with the accession number CCTCC C2012122 that specifically binds to the B-Raf V600K protein but does not bind to the wild-type B-Raf protein.
Lane 1 contains There is a wild-type B-Raf protein expressed by E. coli. Lane 2 contains the mutant B-Raf V600K protein expressed in E. coli. Lane 3 contains the wild-type B-Raf protein expressed by HEK293T cells. Lane 4 contains the mutant B-Raf V600K protein expressed by HEK293T cells.
IF
Figure 2 The immunofluorescence assay map of the antibody produced by the cell line with the accession number CCTCC C2012112 that specifically binds to the B-Raf N581S protein but does not bind to the wild-type B-Raf protein.
The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf N581S protein (lower), respectively. The red fluorescent signal is capable of indicating the binding of the antibody to the B-Raf protein and appears only in cells expressing B-Raf N581S protein (below).
IF
Figure 3 The antibody-specific binding of B-Raf produced by a cell line with the accession number CCTCC C2012122.
The V600K protein does not bind to the immunofluorescence assay of wild-type B-Raf protein. The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf V600K protein (lower), respectively. The red fluorescent signal is indicative of binding of the antibody to the B-Raf protein and appears only in cells expressing the B-Raf V600K protein
Specifications
- Immunogen
- Mutant B-Raf polypeptide
- Host Species
- Mouse
- Specificity
- Human
- Clone
- CCTCC C2012122
- Applications
- IB, IF
- Related Disease
- Skin cancer and other various cancers
Applications
- Application Notes
- This antibody has been tested for use in Immunoblotting and Immunofluorescence.
Target
- Alternative Names
- BRAF; B-Raf proto-oncogene, serine/threonine kinase; NS7; BRAF1; RAFB1; B-RAF1; B-Raf
- Gene ID
- 673
- UniProt ID
- P15056
Related Resources
cAMP Signaling Pathway
MAPK Signaling Pathway
Throid Cancer
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone CCTCC C2012122"
See other products for "BRAF"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-122-F(E) | Recombinant Human Anti-human BRAF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-S(P) | Recombinant Anti-human BRAF Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-122-S(P) | Recombinant Human Anti-human BRAF Antibody scFv Fragment | WB, Neut, FuncS | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1284CL-S(P) | Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-F(E) | Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122) | IB, IF |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0876MZ | Mouse Anti-BRAF Recombinant Antibody (clone 2I23) | ELISA, IHC-P, WB | Mouse IgG1 |
| ZG-0321F | Mouse Anti-BRaf Recombinant Antibody (ZG-0321F) | WB | Mouse IgG |
| ZG-0572J | Rabbit Anti-BRAF Recombinant Antibody (clone 6G3) | ELISA, IHC | Rabbit IgG |
| VS3-CJ659 | Rabbit Anti-BRAF Recombinant Antibody (VS3-CJ659) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-CJ660 | Rabbit Anti-BRAF (phospho T401) Recombinant Antibody (VS3-CJ660) | WB, ICC, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0379 | Hi-Affi™ Rabbit Anti-BRAF Recombinant Antibody (clone DS379AB) | WB, IHC | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0457 | PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0458 | APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0459 | FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0460 | B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0461 | PE-B*27:05/Human BRAF (GRFGLATVK) MHC Tetramer | FCM, IHC |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for TAB-1284CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.