Recombinant Mouse Anti-BfAChE Antibody (Fab410) (CAT#: PABL-375)

Recombinant Mouse Antibody (Fab410) is capable of binding to BfAChE , expressed in Chinese Hamster Ovary cells (CHO).This antibody could inhibit EeAChE and BfAChE by binding to their peripheral sites.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 SDS-PAGE analysis of AChE antibody Fab410.

Figure 1 SDS-PAGE analysis of AChE antibody Fab410.

Electrophoresis patterns obtained by SDS-PAGE in non-reducing conditions (12.5% PhastGel, left) and native-PAGE (7.5% PhastGel, center) with migration from the cathode (top) toward the anode (bottom), and by isoelectric focusing (pI 3-9 PhastGel, right). The three Fabs are more homogenous in mass than in charge, a feature that likely result from variations in the C-terminus generated by papaine cleavage of the CH chain; yet, all isoforms bind EeAChE equally, as verified by a native-PAGE mobility shift assay (not shown). The neutral average pI of Fab408 and cationic average pIs of Fab403 and Fab410 are evident.

Bourne, Y., Renault, L., Essono, S., Mondielli, G., Lamourette, P., Boquet, D., ... & Marchot, P. (2013). Molecular characterization of monoclonal antibodies that inhibit acetylcholinesterase by targeting the peripheral site and backdoor region. PLoS One, 8(10), e77226.


Specifications

  • Immunogen
  • Brachylophus fasciatus acetylcholinesterase
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native B. fasciatus AChE
  • Species Reactivity
  • B. fasciatus
  • Clone
  • Fab410
  • Applications
  • WB 1:1000-1:10000
    ELISA 1:1000-1:20000
    FuncS Assay-depended

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • AChE; acetylcholinesterase

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone Fab410"

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Recombinant Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-004CQ Mouse Anti-ACHE Recombinant Antibody (clone ZR3) Block, IF, IHC, IP Mouse IgG2b

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3945 Hi-Affi™ Rabbit Anti-ACHE Recombinant Antibody (clone SI5DS) ELISA Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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