Human Anti-C5 Recombinant Antibody (clone MEDI7814) (CAT#: PABL-028)

Figure 1 MEDI7814 inhibits fluorescently labelled C5a binding to C5aR1 and C5aR2 receptors.

Representative results showing that MEDI7814 (▲) inhibits Alexa Fluor 647 labelled human C5a (AF647 C5a) binding to human C5aR1 receptor, the isotype matched irrelevant control (■) did not inhibit C5a ligand binding.

Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.

Figure 2 MEDI7814 neutralizes C5a and C5adesArg responses in vitro.

Representative results showing that MEDI7814 (▲) neutralizes calcium mobilisation in HEK 293 expressing human C5aR1 receptor and Ga16 using 100 pM human C5a.

Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.

Figure 3 MEDI7814 is specific for human C5a and C5.

Representative results showing that MEDI7814 does not bind to related human complement proteins: C3a, C3, C4a and C4. Purified complement proteins were titrated into a DELFIA biochemical competition assay measuring MEDI7814 IgG binding to 1.2 nM biotinylated human C5a. MEDI7814 binds to human C5a (●) and C5 (■) as both proteins compete for binding to biotinylated C5a. No competition was observed with complement C3, C3a, C4 and C4a indicating that MEDI7814 does not bind these complement family members when tested at a concentration in excess of 1000- fold over the biotinylated C5a concentration.

Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.