This antibody preferentially binds to cell-associated CA-125/0772P polypeptides relative to shed CA-125/0772P polypeptides, which can be used for preventing, managing, treating or ameliorating one or more symptoms associated with a CA-125/0772P-related disorder and a cell proliferative disorder, such as cancer, e.g., ovarian cancer.
Figure 1 A representative plot from a FACS competition assay of shed CA 125/O772P concentrations versus percent positive cells for, in this instance, 117.1 antibody and Ml 1 antibody control (squares).
Ml 1 can be competed for binding to OVCAR-3 cells even at low concentrations of shed CA 125/O772P (IC50 = 0.003mg/ml) while 117.1 cannot be competed, even at high concentrations of shed CA 125/O772P (IC50 greater than 1 mg/ml).
Figure 2 A representative plot from an ADCC assay of percent lysis versus antibody concentration for 117.1 antibody (average of 4 separate donors).
As shown in the figure, antibody 117.1 mediates specific lysis of OVCAR-3 cells in a dose-dependent manner.
Figure 3 In vivo evaluation of ¹³¹I-labeled 776.1.
NCR nu/nu mice bearing OVCAR-3 tumors were treated with either saline, 100 μCi [¹³¹I]776.1 IgG1, 300 μCi [¹³¹I]776.1 IgG1, or 17 μg unlabeled 776.1 IgG1 (same protein dose as in the 300 μCi [¹³¹I]776.1 IgG1 group). Treatment was a single dose administered intravenously on day 0. The specific activity of [¹³¹I]776.1 was 15 mCi/mg with an immunoreactivity of 51% post- labeling. Results are shown as mean tumor volume+/-SD for 10 mice total per group. Mean tumor size at the beginning of treatment was 199 mm³ for all groups.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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