Mouse Anti-CA9 Recombinant Antibody (clone M75) (CAT#: PABZ-017)

Recombinant Mouse Antibody (M75) is capable of binding to CA9. The adhesion site of CA IX appears to overlap with the PGEEDLP epitope of the monoclonal antibody M75 since the antibody blocks attachment of cells to immobilized CA IX protein.


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  • COA

Figure 1 Strong membrane-stained CA-9 expression (×200).

Figure 1 Strong membrane-stained CA-9 expression (×200).

CA-9 staining was present in 74 of the 101 cancers (73.3%) with no positive staining in any of the control sections.

Rasheed, S., Harris, A. L., Tekkis, P. P., Turley, H., Silver, A., McDonald, P. J., ... & Guenther, T. (2009). Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. Pathology-Research and Practice, 205(1), 1-9.

Figure 2 PAGE and Western blot analysis of MN/CA IX protein.

Figure 2 PAGE and Western blot analysis of MN/CA IX protein.

Lanes 1–3 = gel stained with Coomassie Blue; lanes 4–8 = blots developed with mAb M75 and immunoperoxidase. Lane 1 = Mr reference proteins; lane 2 = purified MN/CA IX ∆TM, IC, 1.25 µg; lane 3 = purified MN/CA IX+, 2 µg; lane 4 = purified MN/CA IX ∆TM, IC, 0.125 µg; lane 5 = purified MN/CA IX+, 0.2 µg; lane 6 = extract from HT29 cells, 50 µl; lane 7 = extract from CGL3 cells, 50 µl; lane 8 = extract from HeLa cells, 50 µl

Zavada, J., Zavadova, Z., Pastorek, J., Biesova, Z., Jezek, J., & Velek, J. (2000). Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion. British journal of cancer, 82(11), 1808.

Figure 3 Competition ELISA with proteins or oligopeptides related to MN/CA IX protein.

Figure 3 Competition ELISA with proteins or oligopeptides related to MN/CA IX protein.

Competing proteins/peptides: (A–C) Recombinant proteins. (A) MN/CA IX+; (B) MN/CA IX∆; (C) GST-MN; (D–G) overlapping proteins covering the PG region: (D) GGSSGEDDPLGEEDLPSEEDSPC (aa 51–72); (E) GEEDLPSEEDSPREEDPPGEEDLPGEC (aa 61–85); (F) EDPPGEEDLPGEEDLPGEEDLPEVC (aa 75–98); (G) EVKPKSEEEGSLKLE (aa 97–111); (H–M) dodecapeptides: (H) GEEDLPGEEDLP; (I) EEDLPGEEDLPG; (J) EDLPGEEDLPGE; (K) DLPGEEDLPGEE; (L) LPGEEDLPGEED; (M) PGEEDLPGEEDL; (N, O) oligopeptides 7 + 2 aa: (N) APGEEDLPA; (O) AGEEDLPGA. Other oligopetides tested which proved negative in competition at 100 µg ml–1 were oligopeptides 7 + 2 aa: AEEDLPGEA, AEDLPGEEA, ADLPGEEDA, ALPGEEDLA and all of the 6 + 2 aa permutations (AGEEDLPA, AEEDLPGA, AEDLPGEA, ADLPGEEA, ALPGEEDA and APGEEDLA). In oligopeptides D, E, F and G the C-terminal amino acid was present as acid, in all others as amide

Zavada, J., Zavadova, Z., Pastorek, J., Biesova, Z., Jezek, J., & Velek, J. (2000). Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion. British journal of cancer, 82(11), 1808.

Figure 4 Blocking of cell attachment by M75

Figure 4 Blocking of cell attachment by M75

Adhesion of cells to the dots of MN/CA IX protein immobilized in bacteriological Petri dishes. This plasticware (not treated for use with tissue cultures) allows no adhesion of CGL1 cells and a limited adhesion of HeLa. (A) CGL1 cells do attach and spread to the dot of adsorbed MN/CA IX. (B) Treatment of the dot with MAb M75 abrogates its binding capacity. (C) Treatment with MAb M16 (irrelevant for MN/CA IX) has no effect on cell attachment. (D) HeLa cells attach to complete MN/CA IX+. (E) HeLa cells do not attach to truncated MN/CA IX∆

Zavada, J., Zavadova, Z., Pastorek, J., Biesova, Z., Jezek, J., & Velek, J. (2000). Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion. British journal of cancer, 82(11), 1808.

Figure 5 The double immunofluorescence staining

Figure 5 The double immunofluorescence staining

The double immunofluorescence images of CA IX (green) and CA II (red) in the human (D) gastric pit and (E ) gastric gland areas show that both proteins occur together in the (D) surface epithelial cells and (E ) parietal cells (arrows). The yellow indicates a colocalization of the proteins.

Pastorekova, S. I. L. V. I. A., Parkkila, S. E. P. P. O., Parkkila, A. K., Opavsky, R. E. N. É., Zelnik, V., Saarnio, J., & Pastorek, J. (1997). Carbonic anhydrase IX, MN/CA IX: analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts. Gastroenterology, 112(2), 398-408.


Specifications

  • Immunogen
  • Human carbonic anhydrase IX
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • CA9
  • Species Reactivity
  • Human
  • Clone
  • M75
  • Applications
  • WB, FuncS

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • CA9; carbonic anhydrase IX; MN; CAIX; carbonic anhydrase 9; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Chimeric Antibody

CAT Product Name Application Type
TAB-707 Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1 - kappa
TAB-H43 Anti-Human CA9 Recombinant Antibody (Iodine (124I) Girentuximab) ELISA, IP, IF, Inhib IgG1 - kappa

Human Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0427 Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) IHC-P, WB Rabbit IgG

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H43 Afuco™ Anti-CA9 ADCC Recombinant Antibody (Girentuximab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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