This product is a recombinant Human antibody that recognizes CA9. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in WB
Western blot analysis of HPAB-0360-CN was performed by loading Human CA9 Protein (His Tag) in reduced condition (Lane 1) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0360-CN (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 2 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in DB
Dot Blot analysis of HPAB-0360-CN was performed by coating with Human CA9 Protein (His Tag).
HPAB-0360-CN incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
Figure 3 Anti-CA9 Recombinant Antibody (HPAB-0360-CN) in ELISA
ELISA analysis of HPAB-0360-CN was performed by coating with Human CA9 Protein (His Tag). Then blocked with BSA and incubated with HPAB-0360-CN. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-707 | Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
TAB-H43 | Anti-Human CA9 Recombinant Antibody (Iodine (124I) Girentuximab) | ELISA, IP, IF, Inhib | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
PABZ-017 | Mouse Anti-CA9 Recombinant Antibody (clone M75) | WB, FuncS | Mouse IgG2b |
MOB-0310CT | Mouse Anti-CA9 Recombinant Antibody (clone 3E4) | ELISA, FC, IF, IHC-P, WB | Mouse IgG1 |
HPAB-0355-CN | Mouse Anti-CA9 Recombinant Antibody (HPAB-0355-CN) | WB, ELISA, FC | Mouse IgG |
VS3-WK1020 | Mouse Anti-CA9 Recombinant Antibody (VS3-WK1020) | WB, IHC, IP | Mouse IgG |
VS3-WK1021 | Rabbit Anti-CA9 Recombinant Antibody (VS3-WK1021) | WB, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-017 | Mouse Anti-CA9 Recombinant Antibody (clone M75); scFv Fragment | WB, FuncS | Mouse scFv |
TAB-1458CL-S(P) | Anti-Human CA9 Recombinant Antibody (MSC3); scFv Fragment | FC | Human scFv |
HPAB-0104-LSX-S(P) | Human Anti-CA9 Recombinant Antibody; scFv Fragment (HPAB-0104-LSX-S(P)) | ELISA | Human scFv |
HPAB-1174WJ-S(P) | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.5.2); scFv Fragment | ELISA | Mouse scFv |
HPAB-1175WJ-S(P) | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.6.1); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1454CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (G250) | Cyt | |
TAB-1455CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (S239D/I332E) | SPR | |
TAB-1456CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC1) | ELISA, FC, Inhib | Fully human antibody |
TAB-1457CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC2) | ELISA, FC, Inhib | Fully human antibody |
TAB-1459CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC4) | ELISA, FC, Inhib | Fully human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1458CL-F(E) | Anti-Human CA9 Recombinant Antibody (MSC3); Fab Fragment | FC | Human Fab |
HPAB-0104-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0104-LSX-F(E)) | ELISA | Human Fab |
HPAB-0105-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0105-LSX-F(E)) | ELISA | Human Fab |
HPAB-0355-CN-F(E) | Mouse Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0355-CN-F(E)) | WB, ELISA, FC | Mouse Fab |
HPAB-0356-CN-F(E) | Mouse Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0356-CN-F(E)) | WB, ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0427 | Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) | IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H43 | Afuco™ Anti-CA9 ADCC Recombinant Antibody (Girentuximab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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