Anti-Human CA9 Recombinant Antibody (S239D/I332E) (CAT#: TAB-1455CL)

This anti-CA9 antibody, S239D/I332E, is a variant version of G250 with amino acid modifications that enhance effector function.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Binding of select anti-CD20Fc variants to human R131FcγRIIa as measured by competition AlphaScreen™ assay.

Figure 1 Binding of select anti-CD20Fc variants to human R131FcγRIIa as measured by competition AlphaScreen™ assay.

In the presence of competitor antibody (Fc variant or WT) a characteristic inhibition curve is observed as a decrease in luminescence signal. The binding data were normalized to the maximum and minimum luminescence signal for each particular curve, provided by the baselines at low and high antibody concentrations respectively. The curves represent the fits of the data to a one site competition model using nonlinear regression.

Figure 2 Binding of select anti-EGFR Fc variants to human FcγRI as measured by competition AlphaScreen assay.

Figure 2 Binding of select anti-EGFR Fc variants to human FcγRI as measured by competition AlphaScreen assay.

SPR

Figure 3 Surface Plasmon Resonance (SPR) (BIAcore) sensorgrams of binding of select anti-EpCAM Fc variants to human R131FcγRIIa.

Figure 3 Surface Plasmon Resonance (SPR) (BIAcore) sensorgrams of binding of select anti-EpCAM Fc variants to human R131FcγRIIa.

Figure 4 Plot of the negative log of the KD for binding of select anti-EpCAM Fc variants to human FcγRI, R131FcγRIIa, H131 FcγRIIa, FcγRIIb, and V158 FcγRIIIa.

Figure 4 Plot of the negative log of the KD for binding of select anti-EpCAM Fc variants to human FcγRI, R131FcγRIIa, H131 FcγRIIa, FcγRIIb, and V158 FcγRIIIa.

Figure 5 The absolute affinity differences between the activating receptors and the inhibitory receptor FcγRIb.

Figure 5 The absolute affinity differences between the activating receptors and the inhibitory receptor FcγRIb.

The top graph shows the affinity differences between both isoforms of FcγRIIa and FcγRIIb, represented mathematically as [-log(KD)FcγRIIa]-[-log(KD)FcγRIIb]. Black represents logarithmic affinity difference between R131FcγRIIa and FcγRIIb, and gray represents the logarithmic affinity difference between H131 FcγRIIa and FcγRIIb. The bottom graph shows the affinity differences between both isoforms of FcγRIIIa and FcγRIIb, represented mathematically as [-log(KD)FcγRIIIa]-[-log(KD)FcγRIIb]. Black represents logarithmic affinity difference between V158 FcγRIIa and FcγRIIb, and gray represents the logarithmic affinity difference between F158 FcγRIIIa and FcγRIIb.

Figure 6 Plot of the negative log of the KD for binding of 293T-expressed (fucosylated) and Lec13-expressed (defucosylated) anti-EpCAM antibodies to human FcγRI, R131FcγRIIa, H131 FcγRIIa, FcγRIIb, and V158 FcγRIIIa.

Figure 6 Plot of the negative log of the KD for binding of 293T-expressed (fucosylated) and Lec13-expressed (defucosylated) anti-EpCAM antibodies to human FcγRI, R131FcγRIIa, H131 FcγRIIa, FcγRIIb, and V158 FcγRIIIa.

*=the data for binding of WT IgG1 defucosylated to FcγRIIb was not determined due to insufficiency of sample.

Figure 7 Binding of select anti-C30 Fc variants to human V158 FcγRIIIa as measured by competition AlphaScreen assay.

Figure 7 Binding of select anti-C30 Fc variants to human V158 FcγRIIIa as measured by competition AlphaScreen assay.

Figure 8 Plot of the negative log of the KO for binding of human and mouse anti-EGFR Fc variant antibodies to mouse Fc receptors FcγRI, FcγRII (FcγRIIb), FcγRIII and FcγRIV.

Figure 8 Plot of the negative log of the KO for binding of human and mouse anti-EGFR Fc variant antibodies to mouse Fc receptors FcγRI, FcγRII (FcγRIIb), FcγRIII and FcγRIV.


Specifications

  • Host Species
  • Human
  • Specificity
  • Human
  • Clone
  • S239D/I332E
  • Applications
  • SPR
  • Related Disease
  • Cancer

Applications

  • Application Notes
  • This antibody has been tested for use in Surface Plasmon Resonance.

Target

  • Alternative Names
  • MN; CAIX

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone S239D/I332E"

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Recombinant Antibody

CAT Product Name Application Type
MOB-1276z Mouse Anti-CA9 Recombinant Antibody (clone 21C7) ELISA, FC, IHC, WB Mouse IgG1, κ
MOB-0310CT Mouse Anti-CA9 Recombinant Antibody (clone 3E4) ELISA, FC, IF, IHC-P, WB Mouse IgG1
HPAB-0104-LSX Human Anti-CA9 Recombinant Antibody (HPAB-0104-LSX) ELISA, FC, Inhib Human IgG
HPAB-0105-LSX Human Anti-CA9 Recombinant Antibody (HPAB-0105-LSX) ELISA, FC, Inhib Human IgG
HPAB-0782-CN Human Anti-CA9 Recombinant Antibody (clone G119) FC, ELISA, Inhib, Cyt, Bioimaging Human IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-707 Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1 - kappa
TAB-H43 Anti-Human CA9 Recombinant Antibody (Iodine (124I) Girentuximab) ELISA, IP, IF, Inhib IgG1 - kappa

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0427 Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) IHC-P, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H43 Afuco™ Anti-CA9 ADCC Recombinant Antibody (Girentuximab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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