This antibody, which can bind to Carbonic Anhydrase IX (CAIX), is useful in the diagnosis and treatment of cancer and in conditions associated with hypoxia and/or elevated CAIX activity.
Figure 1 Affinity of Fab MSC 8 as monitored by surface plasmon resonance.
Serial dilutions of purified Fab were injected on a rhCAIX-coated CM5 sensor chip. Values were corrected for binding to the reference flow cell. The solid lines represent the theoretical curves for each Fab concentration calculated according to the 1 : 1 binding model. KA, ¾ and KD values calculated by the 1 : 1 binding model are shown in the table.
Figure 2 Confirmation of selectivity of the selected antibodies.
Antibody binding to carbonic anhydrase isoforms II, IV, IX, XII and XIV was analyzed in ELISA on recombinant proteins. ESC1 1 IgG recognizing human fibroblast activation protein served as negative control. Coating was confirmed by staining with a Penta-His antibody. Absorbance at 450 nm (A450) is shown for one representative experiment using MSC 8 IgG. Samples were measured in triplicate. Significance was assessed using a paired two-tailed T-test. **: P < 0.01.
Figure 3 Carbonic anhydrase activity, normalized to a scale where activity measured in HCTl 16-CAIX and HCTl 16-EV membrane fragments are one and zero, respectively.
Asterisks denote significant reduction in activity (MSC5, MSC 10„MSC12 and MSC8 Fab antibodies).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1276z | Mouse Anti-CA9 Recombinant Antibody (clone 21C7) | ELISA, FC, IHC, WB | Mouse IgG1, κ |
HPAB-0104-LSX | Human Anti-CA9 Recombinant Antibody (HPAB-0104-LSX) | ELISA, FC, Inhib | Human IgG |
HPAB-0105-LSX | Human Anti-CA9 Recombinant Antibody (HPAB-0105-LSX) | ELISA, FC, Inhib | Human IgG |
HPAB-0782-CN | Human Anti-CA9 Recombinant Antibody (clone G119) | FC, ELISA, Inhib, Cyt, Bioimaging | Human IgG |
HPAB-0784-CN | Human Anti-CA9 Recombinant Antibody (clone G37) | FC, ELISA, Inhib, FuncS, Cyt, Bioimaging, IHC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-707 | Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
TAB-H43 | Anti-Human CA9 Recombinant Antibody (Iodine (124I) Girentuximab) | ELISA, IP, IF, Inhib | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-017 | Mouse Anti-CA9 Recombinant Antibody (clone M75); scFv Fragment | WB, FuncS | Mouse scFv |
HPAB-0104-LSX-S(P) | Human Anti-CA9 Recombinant Antibody; scFv Fragment (HPAB-0104-LSX-S(P)) | ELISA | Human scFv |
HPAB-0782-CN-S(P) | Human Anti-CA9 Recombinant Antibody (clone G119); scFv Fragment | ELISA, FC | Human scFv |
HPAB-0784-CN-S(P) | Human Anti-CA9 Recombinant Antibody (clone G37); scFv Fragment | ELISA, FC | Human scFv |
HPAB-1175WJ-S(P) | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.6.1); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1454CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (G250) | Cyt | |
TAB-1455CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (S239D/I332E) | SPR | |
TAB-1456CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC1) | ELISA, FC, Inhib | Fully human antibody |
TAB-1457CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC2) | ELISA, FC, Inhib | Fully human antibody |
TAB-1459CL-S(P) | Anti-Human CA9 Recombinant Antibody scFv Fragment (MSC4) | ELISA, FC, Inhib | Fully human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0427 | Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) | IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0104-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0104-LSX-F(E)) | ELISA | Human Fab |
HPAB-0105-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0105-LSX-F(E)) | ELISA | Human Fab |
HPAB-0355-CN-F(E) | Mouse Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0355-CN-F(E)) | WB, ELISA, FC | Mouse Fab |
HPAB-0356-CN-F(E) | Mouse Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0356-CN-F(E)) | WB, ELISA, FC | Mouse Fab |
HPAB-0357-CN-F(E) | Mouse Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0357-CN-F(E)) | WB, ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H43 | Afuco™ Anti-CA9 ADCC Recombinant Antibody (Girentuximab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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