This anti-CD 14 antibody can be used in research, diagnostic, and therapeutic applications.
Figure 1 Whole porcine blood was incubated with 150 mg/ml FITC-conjugated Mil2 (FITC-Mil2) and increasing concentrations of unlabeled rMil2 (s), control IgG2/4 (eculizumab) (N), the original clone Mil2 (d) or mIgG2b isotype control (n).
Remaining FITC-Mil2 binding to the granulocyte population was recorded as median fluorescence intensity of using flow cytometry. Fluorescence intensity in presence of FITC-Mil2 alone was set to 100%.
Lau, C., Gunnarsen, K. S., Høydahl, L. S., Andersen, J. T., Berntzen, G., Pharo, A.,... & Nielsen, E. W. (2013). Chimeric anti-CD14 IGG2/4 hybrid antibodies for therapeutic intervention in pig and human models of inflammation. The Journal of Immunology, 191(9), 4769-4777.
Figure 2 Release of the proinflammatory cytokines IL-1b (A) and TNF (B) from porcine whole blood induced with 1 × 10⁵/ml E. coli strain LE392 in presence of increasing concentrations of rMil2 (s), Mil2 (d), control IgG2/4 (N), or mIgG2b (n). After 120 min incubation, plasma cytokine levels were determined with ELISA, and those measured in absence of any exogenous Ab were set to 100%.
Lau, C., Gunnarsen, K. S., Høydahl, L. S., Andersen, J. T., Berntzen, G., Pharo, A.,... & Nielsen, E. W. (2013). Chimeric anti-CD14 IGG2/4 hybrid antibodies for therapeutic intervention in pig and human models of inflammation. The Journal of Immunology, 191(9), 4769-4777.
Figure 3 Blood slides from samples containing 71.4 mg/ml rMil2 or Mil2 were stained with nuclear stain and investigated by light microscopy.
Pictures were taken at a 1000-fold magnification. WBCs, RBCs, and platelets (thrombocytes [TRCs) are indicated by arrows. The single asterisk in the picture for Mil2 indicates a platelet aggregate with surrounding leukocytes that was not observed in rMil2-treated blood.
Lau, C., Gunnarsen, K. S., Høydahl, L. S., Andersen, J. T., Berntzen, G., Pharo, A.,... & Nielsen, E. W. (2013). Chimeric anti-CD14 IGG2/4 hybrid antibodies for therapeutic intervention in pig and human models of inflammation. The Journal of Immunology, 191(9), 4769-4777.
Figure 4 Effect of rMil2 in combination with C5-inhibitor OmCI on the inflammatory response in porcine blood in vitro.
Porcine whole blood was incubated with E. coli in the presence of PBS (E. coli), the complement C5 inhibitor OmCI (0.32 mM), original anti-CD14 Ab Mil2 (25 mg/ml), recombinant anti-CD14 Ab rMil2 (25 mg/ml), the combination of OmCI and the two Mil2 Abs, or an isotype control Ab. Plasma was analyzed for cytokines. (A) TNF. (B) IL-1b. (C) IL-8. (D) TF expression on granulocytes was measured by flow cytometry and expressed as median fluorescence intensity (MFI). Results are shown as mean 6 SEM of four to eight experiments. # Statistical significance between combined inhibition versus the two single inhibitions (for detailed p values, see Results). *Statistical significance as compared with E. coli alone (left column). *p,0.05, **p, 0.01, ***p, 0.001.
Lau, C., Gunnarsen, K. S., Høydahl, L. S., Andersen, J. T., Berntzen, G., Pharo, A.,... & Nielsen, E. W. (2013). Chimeric anti-CD14 IGG2/4 hybrid antibodies for therapeutic intervention in pig and human models of inflammation. The Journal of Immunology, 191(9), 4769-4777.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1182z | Mouse Anti-CD14 Recombinant Antibody (clone 13B10) | ICC, IF, FuncS | Mouse IgG1 |
MOR-0005-FY | Rat Anti-CD14 Recombinant Antibody (clone Sa14-2) | FC, FuncS, IF, IP, WB | Rat IgG2a |
ZG-0837F | Mouse Anti-CD14 Recombinant Antibody (clone FWKW) | FC | Mouse IgG2b |
ZG-0164U | Mouse Anti-CD14 Recombinant Antibody (clone 1A3B6) | ELISA, IHC | Mouse IgG2b |
VS3-CJ502 | Mouse Anti-CD14 Recombinant Antibody (clone ABT-CD14) | IHC-P | Mouse IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1585CL | Anti-Human CD14 Recombinant Antibody (rl8D11) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-1586CL | Anti-Human CD14 Recombinant Antibody (rMil2) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-1588CL | Anti-Human CD14 Recombinant Antibody (Hm2F9) | FC | Chimeric antibody (mouse/human) |
TAB-1590CL | Anti-Human CD14 Recombinant Antibody (F1024) | FuncS | Chimeric antibody (mouse/human) |
TAB-1591CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (F1031-13-2) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1589CL | Anti-Human CD14 Recombinant Antibody (scFv2F9) | WB, FC, Block | Human antibody |
TAB-1589CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (scFv2F9) | WB, FC, Block | Human antibody |
TAB-1589CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (scFv2F9) | WB, FC, Block | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0099MZ | Chicken Anti-CD14 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-277CQ | Human Anti-CD14 Recombinant Antibody (clone D3B8) | Neut, FC | Human IgA2 |
NEUT-279CQ | Mouse Anti-CD14 Recombinant Antibody (clone 4H53) | WB, Neut | Mouse IgG1 |
NEUT-280CQ | Mouse Anti-CD14 Recombinant Antibody (clone 61D3) | FC, IHC-Fr, IP, Neut | Mouse IgG1 |
NEUT-281CQ | Mouse Anti-CD14 Recombinant Antibody (clone CBL693) | WB, FC, CyTOF, Neut | Mouse IgG |
NEUT-283CQ | Mouse Anti-CD14 Recombinant Antibody (clone M5E2) | CyTOF, FC, FuncS, ICC, IF, IHC, Neut | Mouse IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-278CQ | Mouse Anti-CD14 Recombinant Antibody (clone UCHM-1) | FC, IHC, ICC, IP, ELISA, Block, Depletion | Mouse IgG2a, κ |
NEUT-282CQ | Mouse Anti-CD14 Recombinant Antibody (clone BiG10) | FC, ELISA, Inhib, WB | Mouse IgG |
NEUT-284CQ | Rat Anti-CD14 Recombinant Antibody (clone M14-23) | FC, Block | Rat IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0532 | Hi-Affi™ Rabbit Anti-CD14 Recombinant Antibody (clone DS532AB) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0101-YC-S(P) | Mouse Anti-CD14 Recombinant Antibody (clone 28C5); scFv Fragment | ELISA, FC, Block | Mouse scFv |
HPAB-N0031-YC-S(P) | Human Anti-CD14 Recombinant Antibody (clone P1A8); scFv Fragment | IF, WB | Human scFv |
HPAB-1538WJ-S(P) | Mouse Anti-CD14 Recombinant Antibody; scFv Fragment (clone 18E12) | ELISA, WB, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0101-YC-F(E) | Mouse Anti-CD14 Recombinant Antibody (clone 28C5); Fab Fragment | ELISA, FC, Block | Mouse Fab |
HPAB-N0031-YC-F(E) | Human Anti-CD14 Recombinant Antibody (clone P1A8); Fab Fragment | IF, WB | Human Fab |
HPAB-1538WJ-F(E) | Mouse Anti-CD14 Recombinant Antibody; Fab Fragment (clone 18E12) | ELISA, WB, FC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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