Anti-Human CD19 Recombinant Antibody (MDX-1342) (CAT#: TAB-108CL)

Recombinant Human monoclonal antibody expressed in CHO binding to Human CD19. MDX-1342 is a fully human monoclonal antibody (HuMAb) with enhanced antibody-dependent cellular cytotoxicity (ADCC) effector function, targeting CD19-membrane receptor which is highly expressed on malignant chronic lymphocytic leukemia (CLL) cells.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Oligosaccharide analysis of MDX-1342 and parental anti-CD19 antibody by capillary electrophoresis with laser induced fluorescence detection; assignment of carbohydrate structures by comparison with glycan standards.

Figure 1 Oligosaccharide analysis of MDX-1342 and parental anti-CD19 antibody by capillary electrophoresis with laser induced fluorescence detection; assignment of carbohydrate structures by comparison with glycan standards.

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.

FC

Figure 2 FACS titration of MDX-1342 binding on human FcRγIIIa transfected CHO-S cells (a), human lymphoma cell lines (b), and FcRγIIIa-Val158-transfected and FcRIIIa-Phe158-transfected CHO cells (c)

Figure 2 FACS titration of MDX-1342 binding on human FcRγIIIa transfected CHO-S cells (a), human lymphoma cell lines (b), and FcRγIIIa-Val158-transfected and FcRIIIa-Phe158-transfected CHO cells (c)

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.

ADCC

Figure 3 Comparison of ADCC (a) and phagocytosis (b) mediated by MDX-1342 and controls

Figure 3 Comparison of ADCC (a) and phagocytosis (b) mediated by MDX-1342 and controls

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.

FuncS

Figure 4 Kaplan–Meier plot of the Ramos systemic tumor model treated with MDX-1342 at 0.03, 0.3, 3, and 30 mg/kg

Figure 4 Kaplan–Meier plot of the Ramos systemic tumor model treated with MDX-1342 at 0.03, 0.3, 3, and 30 mg/kg

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.

FC

Figure 5 FACS binding of MDX-1342 to CD40+ human B cells (a) and cynomolgus monkey CD40+ B cells (b), rituximab binding to cynomolgus monkey CD40+ B cells (c), and MDX-1342 binding to CHO cells transfected with cynomolgus monkey CD16 (d)

Figure 5 FACS binding of MDX-1342 to CD40+ human B cells (a) and cynomolgus monkey CD40+ B cells (b), rituximab binding to cynomolgus monkey CD40+ B cells (c), and MDX-1342 binding to CHO cells transfected with cynomolgus monkey CD16 (d)

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.

FC

Figure 6 In vivo B-cell depletion in cynomolgus monkeys: FACS analysis of cynomolgus CD20+ B cells following a single dose of 1 mg/kg MDX-1342 or parental anti-CD19 mAb (a) and cynomolgus CD40+ B cells following a single dose of 0.1 mg/kg MDX-1342, rituximab, or control IgG1 (b)

Figure 6 In vivo B-cell depletion in cynomolgus monkeys: FACS analysis of cynomolgus CD20+ B cells following a single dose of 1 mg/kg MDX-1342 or parental anti-CD19 mAb (a) and cynomolgus CD40+ B cells following a single dose of 0.1 mg/kg MDX-1342, rituximab, or control IgG1 (b)

Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Antibody
  • Species Reactivity
  • Human
  • Applications
  • FC, ADCC, FuncS
  • Related Disease
  • Chronic Lymphocytic Leukemia; Rheumatoid Arthritis

Product Property

  • Purity
  • >90% assessed by SDS-PAGE
  • Storage
  • Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Flow Cytometry, ADCC and Functional Assay.

Target

  • Alternative Names
  • CD19; CD19 molecule; B4; CVID3; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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TAB-1622CL Mouse Anti-CD19 Recombinant Antibody (TAB-1622CL) ELISA, FC, Apop, ADCC, FuncS Mouse IgG
TAB-1623CL Mouse Anti-CD19 Recombinant Antibody (TAB-1623CL) ELISA, FC Mouse IgG
TAB-1624CL Anti-Human CD19 Recombinant Antibody (381) FC, Inhib, ELISA

Humanized Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L047E anti-CD19 immunotoxin HD37 (scFv)-PE Cytotoxicity assay, Functional assay
AGTO-L047D anti-CD19 immunotoxin HD37 (scFv)-DT Cytotoxicity assay, Functional assay
AGTO-L047R anti-CD19 immunotoxin HD37 (scFv)-RTA Cytotoxicity assay, Functional assay
AGTO-L047S anti-CD19 immunotoxin HD37 (scFv)-Sap Cytotoxicity assay, Functional assay
AGTO-L047P anti-CD19 immunotoxin HD37 (scFv)-PAP Cytotoxicity assay, Functional assay

Low- or Non-fucosylated Oligosaccharide

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0100MZ Chicken Anti-CD19 Polyclonal IgY WB Chicken antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-286CQ Mouse Anti-CD19 Recombinant Antibody (clone SJ25-C1) Neut, FC, IHC, IHC-Fr, IP Mouse IgG1
NEUT-287CQ Mouse Anti-CD19 Recombinant Antibody (clone HIB19) FC, CyTOF, IHC, Block Mouse IgG1, κ

Blocking Antibody

CAT Product Name Application Type
NEUT-288CQ Rat Anti-CD19 Recombinant Antibody (clone 1D3) Depletion, Block, FC Rat IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0538 Hi-Affi™ Rabbit Anti-CD19 Recombinant Antibody (clone DS538AB) IHC-P Rabbit IgG

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-174 Afuco™ Anti-CD19 ADCC Recombinant Antibody (Taplitumomab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

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