Recombinant Human Anti-CD20 Antibody (C2H7) (CAT#: PABX-026)

Anti-CD20 antibody is a Chimeric antibody (mouse/human) of IgG1 λ class that binds to an CD20. This antibody can mediate complement dependent cytotoxicity and antibody-dependent cellular cytotoxicity effects on CD20 positive human Burkitt lymphoma cells and the Fab fragment can well recognize and bind to an epitope peptide of the extracellular loop of CD20.


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  • Published Data
  • Gene Expression
  • Datasheet
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FuncS

Figure 1 Cytotoxicity assays of C2H7 and Rituximab on Raji cells.

Figure 1 Cytotoxicity assays of C2H7 and Rituximab on Raji cells.

CDC assays with 10% normal human serum as a source of complement and the activities were determined by measuring the amount of released LDH with the CytoTox 96 non-Radioactive Cytotoxicity Assay kit. All experiments were performed in triplicate and the data are presented as mean ±S.D.

Du, J., Wang, H., Zhong, C., Peng, B., Zhang, M., Li, B., ... & Ding, J. (2008). Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide. Molecular immunology, 45(10), 2861-2868.

FuncS

Figure 2 Cytotoxicity assays of C2H7 and Rituximab on Raji cells.

Figure 2 Cytotoxicity assays of C2H7 and Rituximab on Raji cells.

ADCC assays with human peripheral blood mononuclear cells as effectors were performed with respective antibodies on Raji cells and the activities were determined by measuring the amount of released LDH with the CytoTox 96 non-Radioactive Cytotoxicity Assay kit. All experiments were performed in triplicate and the data are presented as mean ±S.D.

Du, J., Wang, H., Zhong, C., Peng, B., Zhang, M., Li, B., ... & Ding, J. (2008). Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide. Molecular immunology, 45(10), 2861-2868.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human CD20
  • Species Reactivity
  • Human
  • Clone
  • C2H7
  • Applications
  • FuncS

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • This antibody has been reported for use in Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

  • Alternative Names
  • MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Humanized Antibody

CAT Product Name Application Type
TAB-133 Anti-Human CD20 Recombinant Antibody (Afutuzumab) IP, IF, FuncS, FC, Neut, ELISA, ICC IgG3 - kappa
TAB-537CL Human Anti-MS4A1 Recombinant Antibody (TAB-537CL) ELISA Human IgG
TAB-1638CL Human Anti-MS4A1 Recombinant Antibody (TAB-1638CL) ADCC, CDC Humanized IgG
TAB-1639CL Human Anti-MS4A1 Recombinant Antibody (TAB-1639CL) ADCC, CDC, Apop Humanized IgG
TAB-1640CL Human Anti-MS4A1 Recombinant Antibody (TAB-1640CL) ADCC, CDC, Apop, FuncS Humanized IgG

Hyper-galactosylated

High-mannose Glycoform

CAT Product Name Application Type
Gly-155LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Humanized antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-171LC Recombinant Anti-Human MS4A1 Antibody (Non-glycosylated) ELISA Chimeric antibody (mouse/human)

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1751CQ Mouse Anti-MS4A1 Recombinant Antibody (clone CBLS-211) Neut, FuncS Mouse IgG1, κ

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-772 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocrelizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-016 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Rituximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-028 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ofatumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-207 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ublituximab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-771 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocaratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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