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anti-CD22 immunotoxin RFB4 (scFv)-PE (AGTO-L048E)

This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.      

Specifications
Host
Mouse
Species Reactivity
Human
Clone
RFB4
Molecule Class
scFv-PE
Toxin Description
Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
Related Disease
Leukemia
Target
Alternative Names
CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2
Entrez Gene ID
12483
UniProt ID
P35329

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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