Anti-Human CD274 Recombinant Antibody (B50-6) (CAT#: TAB-0999CL)
This human monoclonal antibody recognizing PD-L1 was screened by the yeast display technology. The binding affinity of this antibody was tested by SPR with a good result. It specifically binds to PD-L1 with an EC₅₀ value of 28 μg/ml in the binding kinetics detection SPR assay. In the competitive ELISA assay detecting the blocking ability of this antibody, it got an IC₅₀ value of 0.6 μg/ml. This human/mouse antibody could be constructed and produced in Fab or scFv formats and purified by affinity chromatography with a purity higher than 95%.
Specific Inquiry
FuncS
Figure 1 Therapeutic effect of anti-PD-L1 antibodies in mouse tumor models.
MC38 cells were inoculated s.c. into the right flank of female C57BL/6 mice. Treatment with B50-6 or isotype control was initiated on day 0 and continued with another 3 doses on days 3, 7, and 10 at 10 mg/kg. Each group had 6–8 mice. Tumor growth was measured and compared twice weekly.
Luan, Y., Chai, D., Peng, J., Ma, S., Wang, M., Ma, H., ... & Qin, S. (2016). A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity. International immunopharmacology, 31, 248-256.
Specifications
- Host Species
- Human
- Derivation
- Yeast display library
- Type
- Human antibody
- Specificity
- Human
- Clone
- B50-6
- Applications
- FuncS
- Related Disease
- Tumors associated with the use of PD-L1
Applications
- Application Notes
- The antibody was validated for Functional Assay. For details, refer to Published Data.
Target
- Alternative Names
- CD274; CD274 molecule; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
- Gene ID
- 29126
- UniProt ID
- Q0GN75
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0190CL | Anti-Human CD274 Recombinant Antibody (PD1-AB1) | FC, ELISA, FuncS | |
| TAB-0998CL | Anti-Human CD274 Recombinant Antibody (BII61-62) | ELISA, FuncS | Human antibody |
| TAB-1000CL | Human Anti-CD274 Recombinant Antibody (TAB-1000CL) | Inhib, FC | Human IgG1 |
| TAB-1001CL | Human Anti-CD274 Recombinant Antibody (TAB-1001CL) | ADCC, Block, FuncS | Human IgG |
| TAB-0998CL-F(E) | Anti-Human CD274 Recombinant Antibody Fab Fragment (BII61-62) | ELISA | Human antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0262CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Mouse IgG |
| TAB-0992CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL) | Inhib, FC, SPR | Mouse IgG |
| TAB-0993CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL) | FC, SPR | Mouse IgG |
| TAB-0994CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL) | ELISA, FC | Mouse IgG |
| TAB-0262CL-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0262CL-S(P)) | ELISA, WB, SPR, FuncS | Mouse scFv |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL | Human Anti-CD274 Recombinant Antibody (TAB-0995CL) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
| TAB-0995CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0995CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0263CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0263CL-S(P)) | ELISA, WB, SPR, FuncS | Humanized scFv |
| TAB-0264CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0264CL-S(P)) | ELISA, WB, SPR, Inhib, FuncS | Humanized scFv |
| TAB-0265CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0265CL-S(P)) | ELISA, WB, SPR, FuncS | Humanized scFv |
| TAB-0266CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0266CL-S(P)) | ELISA, WB, SPR, FuncS | Humanized scFv |
| TAB-0267CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0267CL-S(P)) | ELISA, WB, SPR, FuncS | Humanized scFv |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-324CQ | Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6) | IHC-P, FC, Neut, ICC | Mouse IgG2b, κ |
| NEUT-325CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-206) | Neut | Human IgG1, κ |
| NEUT-326CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-207) | ELISA, Neut | Human IgG1, κ |
| NEUT-327CQ | Mouse Anti-CD274 Recombinant Antibody (clone MIH1) | FC, FuncS, Neut, IHC, IF | Mouse IgG1, κ |
| NEUT-330CQ | Rat Anti-CD274 Recombinant Antibody (clone MIH5) | FC, FuncS, Neut, IHC-Fr, WB | Rat IgG2a, λ |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-328CQ | Rat Anti-CD274 Recombinant Antibody (VMC171) | Block, FC | Rat IgG2 |
| NEUT-329CQ | Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2) | FC, IF, Block | Rat IgG2b, κ |
| NEUT-331CQ | Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1) | ELISA, Block | Mouse IgG1, λ |
| NEUT-332CQ | Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009) | Block | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0548 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB) | ELISA | Rabbit IgG |
| MOR-4049 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS) | ELISA | Rabbit IgG |
| MOR-4050 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS) | ICC, IF, FC | Rabbit IgG |
| MOR-4051 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS) | FC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0193-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E)) | ELISA | Humanized Fab |
| HPAB-0194-YC-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0561LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0561LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0562LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0562LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-0563LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0563LY-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0326-YC | Human Anti-CD274 Recombinant Antibody (HPAB-N0326-YC) | ELISA, FuncS | Human IgG |
| HPAB-0561LY | Human Anti-CD274 Recombinant Antibody (HPAB-0561LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
| HPAB-0562LY | Human Anti-CD274 Recombinant Antibody (HPAB-0562LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
| HPAB-0563LY | Human Anti-CD274 Recombinant Antibody (HPAB-0563LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
| HPAB-0564LY | Human Anti-CD274 Recombinant Antibody (HPAB-0564LY) | ELISA, Block | Chimeric (mouse/human) IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0408-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (PDL1-56dAb) | ELISA, FC, BL | Human VHH |
| NBD-0065-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (CBP112D) | ELISA | Camelid VHH |
| NBD-0066-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (KN035) | ELISA, Inihb | Camelid VHH |
| NBD-0067-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (Nb1) | ELISA, FC | Camelid VHH |
| NBD-0068-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (Nb2) | ELISA, FC | Camelid VHH |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-417CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody (Durvalumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| AFC-TAB-418CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody (Atezolizumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, BL | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1486-FY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-1486-FY-F(E)) | ELISA | Human Fab |
| HPAB-1487-FY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-1487-FY-F(E)) | ELISA | Human Fab |
| HPAB-0482-YJ-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0482-YJ-S(P)) | IHC | Mouse scFv |
| HPAB-AP572-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-AP572-YC-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-AP573-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-AP573-YC-S(P)) | ELISA, FC, Block | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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