Anti-Human CD28 Recombinant Antibody (TGN1412) (CAT#: TAB-064LC)
TAB-064LC is a superagonistic monoclonal antibody (mAb) capable of fully activating T-cells by cross-linking CD28 molecules, even without signal 1, which sets it apart from conventional anti-CD28 mAbs. TAB-064LC exhibits a binding affinity to human CD28, with an EC50 value of 4.4 nM. TAB-064LC exhibits a binding affinity to human T cells, with EC50 values measured at 2.5 nM.
Specific Inquiry
ELISA
Figure 1 Anti-CD28 Antibody (TAB-064LC) in ELISA
ELISA analysis of TAB-064LC was performed by coating with Recombinant Human CD28 Protein (11524-HCCH). Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TAB-064LC). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-CD28 Antibody (TAB-064LC) in WB
Western blot analysis of TAB-064LC was performed by loading Recombinant Human CD28 Protein (11524-HCCH) onto a 12% Tris-HCl polyacrylamide gel.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)
SDS-PAGE
Figure 3 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in SDS-PAGE
SDS-PAGE analysis of TAB-064LC, Fc silenced, in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-064LC-FcS was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 4 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in HPLC
The purity of TAB-064LC, Fc silenced, was greater than 95% as determined by SEC-HPLC.
WB
Figure 5 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in WB
Western blot analysis of TAB-064LC, Fc silenced, was performed by loading recombinant human CD28 protein.
TAB-064LC-FcS incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Anti-Human CD28 Recombinant Antibody (TAB-064LC), Fc Silenced, in ELISA
ELISA analysis of TAB-064LC, Fc silenced, was performed by coating with recombinant human CD28 protein. Then blocked with BSA, and incubated with Anti-Human CD28 Antibody (TGN1412), Fc Silenced. The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Clone
- TGN1412
- Applications
- FC, Stim
- Related Disease
- Inflammatory diseases, Autoimmune diseases
Applications
- Application Notes
- This antibody has been tested for use in Stimulation. Please see the Published Data for further information.
Target
Related Resources
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone TGN1412"
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-981 | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment | Agonist, Stim, Neu, FC | Mouse IgG1 F(ab')2, κ |
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-0246-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0246-FY-F(E)) | FC, FuncS, ICC, IHC, IP, WB | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody (clone 2E12); Fab Fragment | WB, ELISA | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-065LC | Human Anti-CD28 Recombinant Antibody (TAB-065LC) | ELISA | Human IgG, κ |
| TAB-066LC | Human Anti-CD28 Recombinant Antibody (TAB-066LC) | FC, IF, ELISA | Human IgG1 |
| TAB-069LC | Anti-Human CD28 Recombinant Antibody (HuTN228) | ELISA, FC | Humanized antibody |
| TAB-064LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (TGN1412) | FC | Humanized antibody |
| TAB-065LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-065LC-S(P)) | ELISA | Human scFv |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0354MZ | Mouse Anti-CD28 Recombinant Antibody (clone DD29.1) | ELISA | Mouse IgG1 |
| FAMAB-0013-CN | Mouse Anti-CD28 Recombinant Antibody (clone AV7) | FC | Mouse IgG |
| FN-032CQ | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10) | ELISA, FC, FuncS | Mouse IgG1, κ |
| HPAB-1489-FY | Human Anti-CD28 Recombinant Antibody (clone 2E12) | WB, ELISA | Human IgG1 |
| VS-0723-WK300 | Rabbit Anti-CD28 Recombinant Antibody (VS-0723-WK300) | ELISA, FC | Rabbit IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031 | Recombinant Mouse Anti-CD28 Antibody (5.11A1) | WB, ELISA, FuncS, Activ | IgG |
| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) | WB, ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) | WB, ELISA, FuncS | scFv |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
| NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
| NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
| NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
| MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 1) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 2) | ELISA | Humanized VH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0132CQ-S(P) | Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment | ELISA, IHC, Block | Human scFv |
| HPAB-0245-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P)) | WB, IP, IF, FC | Mouse scFv |
| HPAB-0246-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0246-FY-S(P)) | FC, FuncS, ICC, IHC, IP, WB | Mouse scFv |
| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody (clone 2E12); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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