Rat Anti-CD38 Recombinant Antibody (clone NIMR-5) (CAT#: NEUT-349CQ)

Figure 1 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in SDS-PAGE

SDS-PAGE analysis of NEUT-349CQ-mIgG1 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in SEC-HPLC

The purity of NEUT-349CQ-mIgG1 was greater than 98% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in DB

Dot Blot analysis of NEUT-349CQ-mIgG1 was performed by coating with mouse CD38 protein (His tag).

NEUT-349CQ-mIgG1 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG Fc as a secondary antibody (1: 2000)

Figure 4 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in ELISA

ELISA analysis of NEUT-349CQ-mIgG1 was performed by coating with mouse CD38 protein (His tag). Then blocked with BSA and incubated with NEUT-349CQ-mIgG1. The HRP conjugated Anti-Mouse IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.