This product is a recombinant Human antibody that recognizes Cluster of differentiation 83. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Human Anti-CD83 Recombinant Antibody (TAB-353LC) in SDS-PAGE
SDS-PAGE analysis of TAB-353LC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-353LC was greater than 95% as determined by SDS-PAGE.
Figure 2 Human Anti-CD83 Recombinant Antibody (TAB-353LC) in HPLC
The purity of TAB-353LC was greater than 95% as determined by SEC-HPLC.
Figure 3 Human Anti-CD83 Recombinant Antibody (TAB-353LC) in WB
Western blot analysis of TAB-353LC was performed by loading recombinant human CD83 protein.
TAB-353LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.6 μg)
Figure 4 Human Anti-CD83 Recombinant Antibody (TAB-353LC) in ELISA
ELISA analysis of TAB-353LC was performed by coating with recombinant human CD83 protein. Then blocked with BSA, and incubated with Human Anti-CD83 Recombinant Antibody (TAB-353LC). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1541z | Mouse Anti-CD83 Recombinant Antibody (clone 36G7) | WB, ELISA, FC, IF, IHC, IP | Mouse IgG2a, κ |
MOB-1606MZ | Recombinant Mouse Anti-Human CD83 Antibody (clone 2I5c) | IHC-P | Mouse antibody |
HPAB-0128-LSX | Human Anti-CD83 Recombinant Antibody (HPAB-0128-LSX) | ELISA, FC | Human IgG |
HPAB-0129-LSX | Human Anti-CD83 Recombinant Antibody (HPAB-0129-LSX) | ELISA, FC | Human IgG |
VS-0723-WK126 | Human Anti-CD83 Recombinant Antibody (VS-0723-WK126) | FC | Chimeric (rabbit/human) IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-355LC | Armenian hamster Anti-CD83 Recombinant Antibody (TAB-355LC) | ELISA, FC, FuncS | Armenian hamster IgG |
TAB-356LC | Armenian hamster Anti-CD83 Recombinant Antibody (TAB-356LC) | ELISA, FC, FuncS | Armenian hamster IgG |
TAB-355LC-S(P) | Armenian hamster Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-355LC-S(P)) | ELISA, FC | Armenian hamster scFv |
TAB-356LC-S(P) | Armenian hamster Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-356LC-S(P)) | ELISA, FC | Armenian hamster scFv |
TAB-357LC-S(P) | Armenian hamster Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-357LC-S(P)) | ELISA, FC | Armenian hamster scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-350LC-S(P) | Human Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-350LC-S(P)) | WB, SPR | Human scFv |
TAB-351LC-S(P) | Human Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-351LC-S(P)) | WB, SPR | Human scFv |
TAB-352LC-S(P) | Human Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-352LC-S(P)) | WB, SPR | Human scFv |
TAB-353LC-S(P) | Human Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-353LC-S(P)) | WB, SPR | Human scFv |
TAB-354LC-S(P) | Human Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-354LC-S(P)) | WB, SPR | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0586 | Hi-Affi™ Rabbit Anti-CD83 Recombinant Antibody (clone DS586AB) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0128-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0128-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
HPAB-0129-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0129-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
HPAB-0130-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0130-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0128-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0128-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
HPAB-0129-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0129-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
HPAB-0130-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0130-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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