Anti-Human CLDN4 Recombinant Antibody (5D12) (CAT#: TAB-506LC)

Recombinant monoclonal antibody to CLDN4. 5D12 is a rat monoclonal antibody that can be potentially used in the treatment of pancreatic cancer and bladder cancer.


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FC

Figure 1 Claudin specificity of 5D12. Human (HT‐1080) and murine (L) cells stably expressing various claudins (CLDNs) were incubated with 5 μg/mL 5D12 or vehicle only and then reacted with fluorescence‐conjugated goat anti‐rat IgG (H + L).

Figure 1 Claudin specificity of 5D12. Human (HT‐1080) and murine (L) cells stably expressing various claudins (CLDNs) were incubated with 5 μg/mL 5D12 or vehicle only and then reacted with fluorescence‐conjugated goat anti‐rat IgG (H + L).

The fluorescence intensity of antibody‐treated cells was detected by flow cytometry. Open and filled histograms represent vehicle‐ and 5D12‐treated cells, respectively.

Efficacy and safety evaluation of claudin‐4‐targeted antitumor therapy using a human and mouse cross‐reactive monoclonal antibody

FC

Figure 2 Epitope analysis of 5D12.

Figure 2 Epitope analysis of 5D12.

(A) Sequence alignment of the first (top) and second (bottom) extracellular domains of hCLDN‐4 and hCLDN‐3. Amino acids perfectly conserved between hCLDN‐4 and hCLDN‐3 are highlighted in black. Amino acids in the white regions of hCLDN‐4 were exchanged for the corresponding hCLDN‐3 amino acid to construct the two domain‐swapped hCLDN‐4 molecules. (B) Expression of the domain‐swapped hCLDN‐4s in HT‐1080 cells was evaluated by western blotting. All hCLDN‐4 constructs were detected by an antibody that recognizes the intracellular domain of CLDN‐4, and β‐actin expression levels were used as a loading control. (C) HT‐1080 cells expressing either wild‐type hCLDN‐4 or a domain‐swapped hCLDN‐4 (T33S/V41I/T45N and L151V/A153P/S154E/G155A) were incubated with 5 μg/mL 5D12 or 5A5 and then reacted with fluorescence‐conjugated goat anti‐rat IgG (H + L). The fluorescence intensity of antibody‐treated cells was detected by flow cytometry. White, gray, and black histograms represent vehicle‐, 5D12‐, and 5A5‐treated cells, respectively. (D and E) Effect of conformational structure of hCLDN‐4 on the interaction between 5D12 and hCLDN‐4. Either native (intact) or denatured His‐tagged recombinant hCLDN‐4 was spotted onto polyvinylidene fluoride membrane; spots were detected by using either (D) 5D12 or (E) anti‐His tag antibody; mouse and rat IgG served as spot controls and PBS served as the no‐protein control. CLDNs, claudins PBS, phosphate‐buffered saline.

Efficacy and safety evaluation of claudin‐4‐targeted antitumor therapy using a human and mouse cross‐reactive monoclonal antibody


Specifications

  • Immunogen
  • Fusion human CLDN4 expression plasmid
  • Host Species
  • Rat
  • Specificity
  • Human
  • Species Reactivity
  • Mouse
  • Clone
  • 5D12
  • Applications
  • FC
  • Related Disease
  • Pancreatic cancer, Bladder cancer

Product Property

  • Preservative
  • Human
  • Storage
  • CLDN4 (claudin 4)

Applications

  • Application Notes
  • This antibody has been tested for use in Flow Cytometry. Please see the Published Data for further information.

Target

  • Alternative Names
  • CLDN4; claudin 4; CPER; CPE-R; CPETR; CPETR1; WBSCR8; hCPE-R; claudin-4; CPE-receptor; Clostridium perfringens enterotoxin receptor 1; Williams-Beuren syndrome chromosomal region 8 protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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