Recombinant Mouse Anti-CPV Antibody (mAb8) (CAT#: PABW-142)

Recombinant Mouse Antibody (mAb8) is capable of binding to CPV, expressed in Chinese Hamster Ovary cells (CHO).


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ELISA

Figure 1 Binding of the scFv in ELISA to CPV (A) or FPV (B) capsids or to CPV denatured by boiling (C).

Figure 1 Binding of the scFv in ELISA to CPV (A) or FPV (B) capsids or to CPV denatured by boiling (C).

The reactions are compared with the binding of the original MAb of the same antibodies, and in (C) a rabbit serum (Anti-CPV), which recognizes both assembled and denatured capsid proteins, was also included.

Yuan, W., & Parrish, C. R. (2000). Comparison of two single-chain antibodies that neutralize canine parvovirus: analysis of an antibody-combining site and mechanisms of neutralization. Virology, 269(2), 471-480.

FC

Figure 2 Flow cytometric analysis of the binding of CPV capsids andcanine Tf to TRVb cells transiently expressing the feline TfR or receptors with altered transmembrane or cytoplasmic sequences.

Figure 2 Flow cytometric analysis of the binding of CPV capsids andcanine Tf to TRVb cells transiently expressing the feline TfR or receptors with altered transmembrane or cytoplasmic sequences.

Cells were incubated with Cy5-labeled Tf and CPV together for 1h at 37°C,and then after detachment from the tissue culture dishes and fixation,the cell-associated CPV was detected with Cy2-labeled MAb 8. y axis,Tf binding; x axis, capsid binding.

Hueffer, K., Palermo, L. M., & Parrish, C. R. (2004). Parvovirus infection of cells by using variants of the feline transferrin receptor altering clathrin-mediated endocytosis, membrane domain localization, and capsid-binding domains. Journal of virology, 78(11), 5601-5611.

Neut

Figure 3 Neutralization by scFv compared with that by intact IgG of the same Mab.

Figure 3 Neutralization by scFv compared with that by intact IgG of the same Mab.

Titrations were performed in NLFK cells, and the TCID₅₀ remaining after incubation in the presence of dilutions of the antibody are shown.

Yuan, W., & Parrish, C. R. (2000). Comparison of two single-chain antibodies that neutralize canine parvovirus: analysis of an antibody-combining site and mechanisms of neutralization. Virology, 269(2), 471-480.

IF

Figure 4 CPV infection of cells microinjected simultaneously with MAb 8 (5mg/ml) and the infectious plasmid clone of CPV (200 µg/ml).

Figure 4 CPV infection of cells microinjected simultaneously with MAb 8 (5mg/ml) and the infectious plasmid clone of CPV (200 µg/ml).

(A) MAb 8detected using FITC-labeled goat anti-mouse IgG (green) and viral infectionvisualized with TxR-labeled anti-NS1 antibody (red). The insert shows anothercell subjected to the same treatment. (B) Capsids were present within the ag-gregates seen in the cells. Injected MAb 8 was detected using FITC-labeled goatanti-mouse IgG (green), while capsids were detected using rabbit polyclonalanti-capsid IgG and TxR-conjugated anti-rabbit antibody (red). The insert showsanother cell subjected to the same treatment.

Vihinen-Ranta, M., Yuan, W., & Parrish, C. R. (2000). Cytoplasmic trafficking of the canine parvovirus capsid and its role in infection and nuclear transport. Journal of virology, 74(10), 4853-4859.


Specifications

  • Immunogen
  • CPV
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native CPV
  • Species Reactivity
  • CPV
  • Clone
  • mAb8
  • Applications
  • Neut, ELISA, FC, IF

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The CPV antibody has been reported in applications of Neutralization, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence.

Target

  • Alternative Names
  • CPV; Canine parvovirus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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