Anti-CSF1 ADCC Enhanced Antibody (PD-0360324) is an ADCC enhanced antibody produced by our Afuco™ platform. This is a novel neutralizing human monoclonal antibody against monocyte/ macrophage colony stimulating factor (M-CSF). It exhibited a nonlinear PK profile associated with rapid suppression of PD markers and was generally well tolerated. The safety and efficacy profile will be investigated further in a multi-dose study in patients with rheumatoid arthritis.
Figure 1 Median serum PD-0360324 concentration-time plot.
Concentration values were set below lower limit of quantification to 0 (lower limit of quantification = 35 ng/ml).
Masek-Hammerman,K.,Peeva E.,Ahmad,A.,Menon,S.,Afsharvand, M., Peng Qu,R.,& Pleasic-Williams, S.(2016).Monoclonal antibody against macrophage colony-stimulating factor suppresses circulating monocytes and tissue macrophage function but does not alter cell infiltration/activation in cutaneous lesions or clinical outcomes in patients with cutaneous lupus erythematosus. Clinical & Experimental Immunology,183(2),258-270.
Figure 2 Monocyte populations and other urinary/serum biomarkers.
Mean percentage of changes from baseline in CD14+ monocytes (a),CD14+ CD16+ monocytes (b) and urinary N-terminal telopeptide (uNTX) (c).P < 0.05; P < 0.01; p< 0·001; P < 0.0001, PD-0360324 versus placebo.
Masek-Hammerman,K.,Peeva E.,Ahmad,A.,Menon,S.,Afsharvand, M., Peng Qu,R.,& Pleasic-Williams, S.(2016).Monoclonal antibody against macrophage colony-stimulating factor suppresses circulating monocytes and tissue macrophage function but does not alter cell infiltration/activation in cutaneous lesions or clinical outcomes in patients with cutaneous lupus erythematosus. Clinical & Experimental Immunology,183(2),258-270.
Figure 3 consistent with previous studies, significantly greater percentages of change from baseline in ALT, AST and CK levels were observed in the active treatment groups compared with the placebo group at most time-points throughout the treatment period.
Mean percentage of changes from baseline in alanine aminotransferase (ALT) (a),aspartate aminotransferase (AST) (b)and creatine kinase (CK) levels (c).P < 0.05; P < 0.01; P < 0.001; P < 0.0001, PD-0360324 versus placebo.
Masek-Hammerman,K.,Peeva E.,Ahmad,A.,Menon,S.,Afsharvand, M., Peng Qu,R.,& Pleasic-Williams, S.(2016).Monoclonal antibody against macrophage colony-stimulating factor suppresses circulating monocytes and tissue macrophage function but does not alter cell infiltration/activation in cutaneous lesions or clinical outcomes in patients with cutaneous lupus erythematosus. Clinical & Experimental Immunology,183(2),258-270.
Figure 4 Relationship between pharmacokinetics and pharmacodynamics.
Mean serum trough PD-0360324 concentrations and mean (a)CD16+ monocytes, urinary N-terminal telopeptide (uNTX):creatinine ratio (b), alanine aminotransferase (ALT) (c),aspartate aminotransferase (AST)(d) and creatine kinase (CK) levels (e).
Masek-Hammerman,K.,Peeva E.,Ahmad,A.,Menon,S.,Afsharvand, M., Peng Qu,R.,& Pleasic-Williams, S.(2016).Monoclonal antibody against macrophage colony-stimulating factor suppresses circulating monocytes and tissue macrophage function but does not alter cell infiltration/activation in cutaneous lesions or clinical outcomes in patients with cutaneous lupus erythematosus. Clinical & Experimental Immunology,183(2),258-270.
Figure 5 Immunohistochemistry results in tissue biopsy samples from completed patients.
Immunohistochemistry results in tissue biopsy samples from completed patients. Representative images of CD68, CD163, human leucocyte antigen D-related (HLA-DR), CD3 and CXCR3 expression in baseline and post-treatment (d84) lesional biopsy samples from a patient receiving 100 mg PD-0360324 (a). Area of positive signal for each marker was determined by quantitative image analysis in epidermis or dermis (b). Counts were then normalized to measured tissue area (epidermis or dermis, respectively) and expressed as percentage area of positive signal. Results are presented for patients receiving placebo (grey bars) or 100 or 150 mg PD-0360324 (black bars).
Masek-Hammerman,K.,Peeva E.,Ahmad,A.,Menon,S.,Afsharvand, M., Peng Qu,R.,& Pleasic-Williams, S.(2016).Monoclonal antibody against macrophage colony-stimulating factor suppresses circulating monocytes and tissue macrophage function but does not alter cell infiltration/activation in cutaneous lesions or clinical outcomes in patients with cutaneous lupus erythematosus. Clinical & Experimental Immunology,183(2),258-270.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-245-S(P) | Recombinant Anti-human CSF1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
HPAB-0748-WJ-S(P) | Human Anti-CSF1 Recombinant Antibody (clone 88); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0749-WJ-S(P) | Human Anti-CSF1 Recombinant Antibody (clone 100); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0750-WJ-S(P) | Human Anti-CSF1 Recombinant Antibody (clone 3.8.3); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0751-WJ-S(P) | Human Anti-CSF1 Recombinant Antibody (clone 2.7.3); scFv Fragment | ELISA, WB, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-245 | Recombinant Human Anti-human CSF1 Antibody | ELISA, WB, FuncS | IgG |
HPAB-0532-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0532-CN) | ELISA, FC, Neut | Mouse IgG |
HPAB-0533-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0533-CN) | ELISA, FC, Neut | Mouse IgG |
ZG-0274C | Mouse Anti-CSF1 Recombinant Antibody (ZG-0274C) | WB, IHC, ELISA | Mouse IgG |
VS3-QX283 | Mouse Anti-CSF1 Recombinant Antibody (clone 2D10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-187CL | Human Anti-CSF1 Recombinant Antibody (TAB-187CL) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-501CQ | Mouse Anti-CSF1 Recombinant Antibody (clone 3V17) | Neut, WB | Mouse IgG2 |
NEUT-502CQ | Mouse Anti-CSF1 Recombinant Antibody (clone CBL140) | Neut, WB | Mouse IgG2 |
NEUT-503CQ | Mouse Anti-CSF1 Recombinant Antibody (clone CBL441) | WB, Neut | Mouse IgG2a |
NEUT-505CQ | Rat Anti-Csf1 Recombinant Antibody (clone CBL205) | WB, Neut | Rat IgG2 |
NEUT-506CQ | Rat Anti-Csf1 Recombinant Antibody (clone CBL535) | WB, Neut | Rat IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-504CQ | Rat Anti-Csf1 Recombinant Antibody (clone 5A1) | Block | Rat IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0824 | Hi-Affi™ Rabbit Anti-CSF1 Recombinant Antibody (clone DS824AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-029ML | Anti-Human CSF1 Recombinant Antibody (Lacnotuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0532-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0532-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
HPAB-0533-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0533-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
HPAB-0534-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0534-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
HPAB-0150CQ-F(E) | Human Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0150CQ-F(E)) | ELISA, WB, FC, Inhib | Human Fab |
HPAB-0151CQ-F(E) | Human Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0151CQ-F(E)) | ELISA, WB, FC, Inhib | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-029ML | Afuco™ Anti-CSF1 ADCC Recombinant Antibody (Lacnotuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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