Anti-Human CXCL12 Recombinant Antibody (30D8) (CAT#: TAB-415CT)

Recombinant monoclonal antibody against mouse and human CXCL12α, CXCL12β, and CXCL12γ, was shown to dose-dependently block CXCL12α binding to CXCR4 and CXCR7, and CXCL12α-induced Jurkat cell migration in vitro.


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Inhib

Figure 1 Characterization of hamster anti-CXCL12 antibodies.

Figure 1 Characterization of hamster anti-CXCL12 antibodies.

Dose-dependent inhibition of CXCL12α-induced Jurkat cell migration in the absence or presence of various concentrations of hamster antibodies. Of note, 10 ng/mL human CXCL12α was preincubated with antibodies in serial 1:3 dilutions at room temperature for 30 minutes. The highest concentration used for each antibody is shown. IC₅₀ was the average of 3 independent experiments. Data are expressed as mean ± SD.

Zhong, C., Wang, J., Li, B., Xiang, H., Ultsch, M., Coons, M., ... & Quintana, L. (2013). Development and preclinical characterization of a humanized antibody targeting CXCL12. Clinical Cancer Research, 19(16), 4433-4445.

WB

Figure 2 Characterization of hamster anti-CXCL12 antibodies.

Figure 2 Characterization of hamster anti-CXCL12 antibodies.

Effects of 30D8 on CXCL12α-induced Jurkat cell Rac activation. Jurkat cells were treated with 100 ng/mL human CXCL12α for 10 minutes in the presence of various concentrations of 30D8, 46H9, 18E9, or control hamster antibody, MAB170 or AMD3100, as indicated. The total and GTP-bound Rac1 were detected by Western blotting using an antibody against Rac1. Data were representative of 3 independent studies.

Zhong, C., Wang, J., Li, B., Xiang, H., Ultsch, M., Coons, M., ... & Quintana, L. (2013). Development and preclinical characterization of a humanized antibody targeting CXCL12. Clinical Cancer Research, 19(16), 4433-4445.

FC

Figure 3 Effects of 30D8 in various mouse tumor models, alone or in combination with anti-VEGF antibody B20.4.1.1 (B20).

Figure 3 Effects of 30D8 in various mouse tumor models, alone or in combination with anti-VEGF antibody B20.4.1.1 (B20).

Percentage of F4/80+, CD11b+/Gr1+, and CD31+ cells in EL4 tumors treated with control antibody, 30D8, or B20.4.1.1 at day 10 after tumor cell implantation using FACS. Five tumors per treatment group were used and stained with appropriate antibodies.

Zhong, C., Wang, J., Li, B., Xiang, H., Ultsch, M., Coons, M., ... & Quintana, L. (2013). Development and preclinical characterization of a humanized antibody targeting CXCL12. Clinical Cancer Research, 19(16), 4433-4445.

Funcs

Figure 4 Effects of 30D8 in various mouse tumor models, alone or in combination with anti-VEGF antibody B20.4.1.1 (B20).

Figure 4 Effects of 30D8 in various mouse tumor models, alone or in combination with anti-VEGF antibody B20.4.1.1 (B20).

All antibodies were administered intraperitoneally. A, dose-dependent efficacy of 30D8 in the EL4 model. C57BL6 mice (n = 10/group) bearing EL4 tumors were treated with 30D8 at various doses (three times/week), with or without anti-VEGF antibody B20.4.1.1 at 10 mg/kg (twice/week). Hamster antibody gD:2566 served as a control antibody for 30D8. Tumor size was measured at day 3, 5, 8, 10, and 12.

Zhong, C., Wang, J., Li, B., Xiang, H., Ultsch, M., Coons, M., ... & Quintana, L. (2013). Development and preclinical characterization of a humanized antibody targeting CXCL12. Clinical Cancer Research, 19(16), 4433-4445.

ELISA

Figure 5 Pharmacokinetic profiles of 30D8.

Figure 5 Pharmacokinetic profiles of 30D8.

30D8 serum concentration–time profiles after a single intraperitoneal administration in Balb/c nude mice at 30 mg/kg (n = 3/time point with a total of n = 9/group). Serum was collected at various time points, up to 7 days after 30D8 administration. Total hamster IgG1 levels were measured by a generic hamster antibody ELISA.

Zhong, C., Wang, J., Li, B., Xiang, H., Ultsch, M., Coons, M., ... & Quintana, L. (2013). Development and preclinical characterization of a humanized antibody targeting CXCL12. Clinical Cancer Research, 19(16), 4433-4445.


Specifications

  • Host Species
  • Hamster
  • Specificity
  • Human
  • Species Reactivity
  • Mouse and Human CXCL12α, CXCL12β, and CXCL12γ
  • Clone
  • 30D8
  • Applications
  • Inhib, WB, FC, Funcs, ELISA, Neut
  • Related Disease
  • Cancer and inflammation-related diseases

Applications

  • Application Notes
  • The CXCL12 antibody has been reported in applications of Inhibition, Western Blot, Flow Cytometry, Functional Assay, Enzyme-linked Immunosorbent Assay, Neutralization.

Target

  • Alternative Names
  • CXCL12; chemokine (C-X-C motif) ligand 12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; anti-SDF-1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 30D8"

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Single-domain Antibody

Humanized Antibody

CAT Product Name Application Type
TAB-416CT Anti-Human CXCL12 Recombinant Antibody (hu30D8) Funcs, FC, PK, ELISA, Neut, WB Humanized antibody
TAB-416CT-S(P) Anti-Human CXCL12 Recombinant Antibody scFv Fragment (hu30D8) WB, ELISA, Neut, FC Humanized antibody
TAB-416CT-F(E) Anti-Human CXCL12 Recombinant Antibody Fab Fragment (hu30D8) WB, ELISA, Neut, FC Humanized antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-612CQ Mouse Anti-CXCL12 Recombinant Antibody (clone CBL825) Neut Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0884 Hi-Affi™ Rabbit Anti-CXCL12 Recombinant Antibody (clone DS884AB) WB, ICC, IF Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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