Recombinant Mouse Antibody (F10. 6. 6) is capable of binding to Chicken LYZ. This fab was obtained after long-term exposure to the antigen and belongs to a group of antibodies, including fab D44. 1, which recognize the β-pleated sheet domain of HEL.
Figure 1 Enhancement of HEL presentation to a specific T cell hybridoma by some anti-HEL mAb.
The L3E10 T cell hybridoma (anti-HEL103–117, I-Eᵈ) and the A6B9 B cell lymphoma (FcγRIIb2⁺) were incubated with various concentrations of HEL, in the presence or absence of a constant dose (0.17 μM) of different anti-HEL mAb or isotype-matched control mAb (A) or with a constant, sub-optimal dose of HEL (0.1 μM) in the presence of various concentrations of anti-HEL mAb or isotype-matched control mAb (B). IL-2 released by the hybridoma was assessed by the proliferation of the CTLL cell line. Results are expressed in c.p.m.
Guermonprez, P., Lo-Man, R., Sedlik, C., Rojas, M. J., Poljak, R. J., & Leclerc, C. (1999). mAb against hen egg-white lysozyme regulate its presentation to CD4+ T cells. International immunology, 11(11), 1863-1872.
Figure 2 Differential regulation of presentation of Ii-independent and Ii-dependent HEL epitopes presentation by anti-HEL mAb.
I-Aᵏ-restricted, anti-HEL T cell hybridomas (1D6.1, anti-HEL116–129, in A; 3B11.1, anti-HEL 34–45, in B; 3A9, anti-HEL 48–61, in C) and the A6B9.k cells (FcγRIIb2⁺, I-Aᵏ) were incubated with various concentrations of HEL, in the presence or absence of a constant dose (0.17 μM) of different anti-HEL mAb or isotype-matched control mAb. IL-2 released by the hybridoma was assessed by the proliferation of the CTLL cell line. For each mAb, the HEL dose–response of the T cell hybrid was determined and the enhancing effect on HEL presentation was quantified by the ratio: (HEL dose required for 50% maximal T cell hybridoma IL-2 release without mAb)/(HEL dose required for 50% maximal maximal T cell hybridoma IL-2 release with mAb). For each T cell hybridoma, the enhancing effect ratio (fold) is plotted against the mAb tested. Enhancing effect ratio represents the mean of at least two experiments, each including all experimental points in duplicate.
Guermonprez, P., Lo-Man, R., Sedlik, C., Rojas, M. J., Poljak, R. J., & Leclerc, C. (1999). mAb against hen egg-white lysozyme regulate its presentation to CD4+ T cells. International immunology, 11(11), 1863-1872.
Figure 3 Kinetics and thermodynamics of mAbs D44.1 and F10.6.6 reactions with lysozyme at 25°C
Cauerhff, A., Goldbaum, F. A., & Braden, B. C. (2004). Structural mechanism for affinity maturation of an anti-lysozyme antibody. Proceedings of the National Academy of Sciences, 101(10), 3539-3544.
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10); Fab Fragment | WB, IHC, FuncS | Human Fab |
PFBL-268 | Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
PFBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); Fab Fragment | WB, FuncS | Mouse Fab |
PFBW-128 | Recombinant Mouse Anti-Turkey LYZ Antibody Fab Fragment (1F9) | ELISA | IgG |
PFBL-426 | Human Anti-LYZ Recombinant Antibody; Fab Fragment | WB, IF, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-023 | Recombinant Anti-Human LYZ VHH Single Domain Antibody (cAbHuL5) | WB, ELISA, FuncS | Llama VHH |
PNBL-024 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody () | WB, ELISA, FuncS | Llama VHH |
PNBL-036 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (1D2L19) | WB, FuncS | Llama VHH |
PNBL-051 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAbBCII-10) | ELISA | Llama VHH |
PNBL-052 | Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (cAb-HuL6) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PABW-102 | Mouse Anti-LYZ Recombinant Antibody (clone D11.15) | ELISA | Mouse IgG |
PABL-429 | Human Anti-LYZ Recombinant Antibody | WB, IF, FuncS | Human IgG |
MOB-1014MZ | Mouse Anti-LYZ Recombinant Antibody (clone BGN/06/961) | ELISA, WB | Mouse IgG2a |
MRO-0949-CN | Recombinant Rabbit Anti-LYZ Monoclonal Antibody (CBACN-349) | WB, IF, IHC, IP | Rabbit IgG |
MOB-132YJL | Mouse Anti-LYZ Recombinant Antibody (clone muHYHEL-10) | WB, IHC, FuncS | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
PABX-073-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-074 | Recombinant Mouse Anti-Lysozyme Antibody (HyHEL-5) | WB, ELISA, FuncS | IgG |
PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) | WB, ELISA, FuncS | scFv |
PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2112 | Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) | ICC, IHC-P, IP, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0212CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Hyb.C1) | ELISA | Mouse scFv |
FAMAB-0213CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop2) | ELISA | Mouse scFv |
FAMAB-0214CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop3) | ELISA | Mouse scFv |
FAMAB-0215CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop5) | ELISA | Mouse scFv |
FAMAB-0132-CN-S(P) | Recombinant Humanized Anti-LYZ Antibody scFv Fragment (HuD1.3) | ELISA, IF | Humanized scFv |
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