Mouse Anti-Cocaine benzoyl ester Recombinant Antibody (clone 15A10) (CAT#: PABZ-028)
Recombinant Mouse Antibody (15A10) is capable of binding to Cocaine benzoyl ester, expressed in Chinese Hamster Ovary cells (CHO). Catalytic antibody 15A10 hydrolyzes the benzoyl ester of cocaine to form the nonpsychoactive metabolites benzoic acid and ecgonine methylester. 15A10 hydrolyzes the benzoyl ester of cocaine to yield the nonpsychoactive metabolite ecgonine methylester with a kcat of 2. 3 min-1, KM of 220 μM, and kcatkuncat-1 =2. 3x104.
Specific Inquiry
Figure 1 Log dose-response relationship for mAb 15A10 on rats' survival after infusion of an LD90 (16 mg/kg) cocaine.
The effect of mAb 15A10 on survival was significant by a X2 test (P < 0.001).
Mets, B., Winger, G., Cabrera, C., Seo, S., Jamdar, S., Yang, G., ... & Landry, D. W. (1998). A catalytic antibody against cocaine prevents cocaine’s reinforcing and toxic effects in rats. Proceedings of the National Academy of Sciences, 95(17), 10176-10181.
Figure 2 The effect of Mab 15A10 in a model of addiction
Pattern of intravenous self-administration of cocaine (A), saline (B), or cocaine + mAb 15A10 (C) in a single rat. Each vertical line indicates a single injection, obtained on a fixed ratio 5 time-out 10 sec schedule of cocaine delivery.The panels show infusion patterns from three consecutive sessions.
De Prada, P., Winger, G., & Landry, D. W. (2000). Application of artificial enzymes to the problem of cocaine. Annals of the New York Academy of Sciences, 909(1), 159-169.
Figure 3 Serum was analysed for the presence of antibody using ELISA technique.
Antibody detected in mice serum after the subcutaneous injection of pure antibody (A) or microspheres (B). Data are the average of two mice measured in triplicate ± s.d.
Homayoun, P., Mandal, T., Landry, D., & Komiskey, H. (2003). Controlled release of anti‐cocaine catalytic antibody from biodegradable polymer microspheres. Journal of pharmacy and pharmacology, 55(7), 933-938.
Specifications
- Immunogen
- Cocaine
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- 15A10
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 15A10 has been reported in applications of Functional Assay, Blocking, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Functional Assay: Rats pretreated with mAb 15A10 showed a significant dose-dependent increase in survival after an LD90 cocaine infusion. The concentration of 15A10 was 5 mg/kg-50 mg/kg.
Blocking: It is reported that the administration of Mab 15A10 (9 mg) completely blocked the reinforcing effect of cocaine in rat model.
Target
- Alternative Names
- Methyl (1R; 2R; 3S; 5S)-3-(benzoyloxy)-8-methyl-8 azabicyclo[3.2.1] octane-2-carboxylate; Cocaine; benzoylmethylecgonine; Bernice; Bernies
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-028 | Mouse Anti-Cocaine benzoyl ester Recombinant Antibody (clone 15A10); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-028 | Mouse Anti-Cocaine benzoyl ester Recombinant Antibody (clone 15A10); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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