Anti-DENV-1 antibody is a Mouse antibody of IgG2 class that binds to an DENV-1.
Figure 1 E106 Fab and MAb binds with low affinity to DENV-1-DIII.
Analysis of DENV-1 DIII binding to (A) E106 Fab and (B) E106 MAb as measured by SPR. The kinetically fit sensorgrams (fits in gray, raw data in black) for which a 5.8±3.0 µM affinity (Fab) and 4.2±1.2 µM (MAb) was calculated on the left panel is similar to the equilibrium data fit 4.8±2.1 µM (Fab) and 3.2±0.8 µM (MAb) which is shown on the right panel.
Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.
Figure 2 Fab versus MAb neutralization and binding.
(A) DENV-1 neutralization by E106 MAb (filled square, red) and Fab (empty square, salmon) (upper panel) and corresponding fold reduction (lower panel). The control E103 MAb (filled circle, blue) and corresponding Fab (empty circle, purple) is included for comparison.
Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.
Figure 3 Fab versus MAb neutralization and binding.
(B) Qualitative ELISA binding of MAbs and Fabs to DENV-1. E106 MAb binds virions to a similar extent as E103 MAb but E106 Fab binding is significantly less than E103 Fab (P<0.0001). The negative control is E16. The results are from two ELISA experiments performed in duplicate and without background subtraction. The limit of detection was determined by performing the assay in the absence of virus.
Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.
Figure 4 Binding of MAbs to DIII or virions of 16007 and West Pac-74.
A. MAb binding to DIII of 16007 or West Pac-74 on yeast. Flow cytometry histograms of DIII of 16007 or West Pac-74 (different at residues 339 and 345) with neutralizing MAbs. Data shown are representative of two independent experiments.
Shrestha, B., Brien, J. D., Sukupolvi-Petty, S., Austin, S. K., Edeling, M. A., Kim, T., ... & Diamond, M. S. (2010). The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1. PLoS pathogens, 6(4), e1000823.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-273 | Recombinant Anti-DENV 1 Antibody | ELISA, WB, IP, FuncS | IgG |
MHH-273 | Recombinant Human Anti-DENV 1 Antibody | ELISA, WB, Neut, FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-273-F(E) | Recombinant Anti-DENV 1 Antibody Fab Fragment | WB, IF, FuncS | Fab |
MHH-273-F(E) | Recombinant Human Anti-DENV 1 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-273-S(P) | Recombinant Anti-DENV 1 Antibody scFv Fragment | Neut, IF, FuncS | scFv |
MHH-273-S(P) | Recombinant Human Anti-DENV 1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-046-S (P) | Recombinant Mouse Anti-DENV-1 Antibody scFv Fragment (DENV1-E106) | WB, ELISA, FuncS | scFv |
PABX-046-F (E) | Recombinant Mouse Anti-DENV-1 Antibody Fab Fragment (DENV1-E106) | WB, ELISA, FuncS | Fab |
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