Recombinant Mouse Anti-DENV-1 Antibody (DENV1-E106) (CAT#: PABX-046)

Anti-DENV-1 antibody is a Mouse antibody of IgG2 class that binds to an DENV-1.


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SPR

Figure 1 E106 Fab and MAb binds with low affinity to DENV-1-DIII.

Figure 1 E106 Fab and MAb binds with low affinity to DENV-1-DIII.

Analysis of DENV-1 DIII binding to (A) E106 Fab and (B) E106 MAb as measured by SPR. The kinetically fit sensorgrams (fits in gray, raw data in black) for which a 5.8±3.0 µM affinity (Fab) and 4.2±1.2 µM (MAb) was calculated on the left panel is similar to the equilibrium data fit 4.8±2.1 µM (Fab) and 3.2±0.8 µM (MAb) which is shown on the right panel.

Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.

Neut

Figure 2 Fab versus MAb neutralization and binding.

Figure 2 Fab versus MAb neutralization and binding.

(A) DENV-1 neutralization by E106 MAb (filled square, red) and Fab (empty square, salmon) (upper panel) and corresponding fold reduction (lower panel). The control E103 MAb (filled circle, blue) and corresponding Fab (empty circle, purple) is included for comparison.

Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.

ELISA

Figure 3 Fab versus MAb neutralization and binding.

Figure 3 Fab versus MAb neutralization and binding.

(B) Qualitative ELISA binding of MAbs and Fabs to DENV-1. E106 MAb binds virions to a similar extent as E103 MAb but E106 Fab binding is significantly less than E103 Fab (P<0.0001). The negative control is E16. The results are from two ELISA experiments performed in duplicate and without background subtraction. The limit of detection was determined by performing the assay in the absence of virus.

Edeling, M. A., Austin, S. K., Shrestha, B., Dowd, K. A., Mukherjee, S., Nelson, C. A., ... & Pierson, T. C. (2014). Potent dengue virus neutralization by a therapeutic antibody with low monovalent affinity requires bivalent engagement. PLoS pathogens, 10(4), e1004072.

FC

Figure 4 Binding of MAbs to DIII or virions of 16007 and West Pac-74.

Figure 4 Binding of MAbs to DIII or virions of 16007 and West Pac-74.

A. MAb binding to DIII of 16007 or West Pac-74 on yeast. Flow cytometry histograms of DIII of 16007 or West Pac-74 (different at residues 339 and 345) with neutralizing MAbs. Data shown are representative of two independent experiments.

Shrestha, B., Brien, J. D., Sukupolvi-Petty, S., Austin, S. K., Edeling, M. A., Kim, T., ... & Diamond, M. S. (2010). The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1. PLoS pathogens, 6(4), e1000823.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native DENV-1
  • Species Reactivity
  • Dengue virus-1
  • Clone
  • DENV1-E106
  • Applications
  • WB, FuncS, SPR, Neut, ELISA, FC

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The DENV 1 antibody has been reported in applications of Western Blot, Functional Assay, Surface Plasmon Resonance, Neutralization, Enzyme-linked Immunosorbent Assay, Flow Cytometry.
    For ELISA: Humanized DENV-1 E50 MAb (subcomplex DIII A-strand specific antibody) was coated at 2 µg/ml on MaxiSorp (Nunc) polystyrene 96-well microtiter plates in a sodium carbonate (pH 9.3) buffer. Plates were washed three times in wash buffer (PBS with 0.02% Tween 20) and blocked for one hour at 37°C with blocking buffer (DMEM with 10% FBS). DENV-1 virions (2.5×105 PFU) diluted in DMEM with 10% heat-inactivated FBS were captured on plates for two hours at 37°C. Wells were washed thrice with blocking buffer and DENV-1 MAb or Fab was then added at 100 µg/ml and 4-fold serial dilutions to duplicate wells and incubated for two hours at 37°C. Plates were washed five times and then sequentially incubated with goat anti-mouse (whole molecule) IgG-HRP and tetramethylbenzidine substrate (Dako). The reaction was stopped with the addition of 2 N H2SO4 to the medium, and emission (450 nm) was read using an iMark microplate reader.

Target

  • Alternative Names
  • Dengue virus; DENV; Flaviviridae; dengue virus 1; DENV 1; Flaviviridae

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone DENV1-E106"

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Recombinant Antibody

CAT Product Name Application Type
MOB-273 Recombinant Anti-DENV 1 Antibody ELISA, WB, IP, FuncS IgG
MHH-273 Recombinant Human Anti-DENV 1 Antibody ELISA, WB, Neut, FuncS IgG

Fab Fragment Antibody

scFv Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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