Recombinant Mouse Anti-DENV-4 EDIII Antibody (2H12) (CAT#: PABC-149)
Recombinant Mouse Antibody (2H12) is capable of binding to DENV-4 EDIII, expressed in Chinese Hamster Ovary cells (CHO). 2H12 neutralization varied between dengue serotypes and strains; in particular, dengue serotype 2 was not neutralized.
Specific Inquiry
DB
Figure 1 Mouse Anti-DENV-4 EDIII Antibody (2H12) in DB.
Binding properties of mAb 2H12. Dot blots against recombinant EDIII from all four serotypes.
Midgley, C. M., Flanagan, A., Tran, H. B., Dejnirattisai, W., Chawansuntati, K., Jumnainsong, A., ... & Screaton, G. R. (2012). Structural analysis of a dengue cross-reactive antibody complexed with envelope domain III reveals the molecular basis of cross-reactivity. The Journal of Immunology, 188(10), 4971-4979.
FuncS
Figure 2 Mouse Anti-DENV-4 EDIII Antibody (2H12) in FuncS.
Functional properties of mAb 2H12. Neutralisation assays of 2H12 on three dengue strains from Den1, 2, 3 or 4, expressed as FRNT on infected Vero cells, mAb 4G2, which cross-reacts against the envelope of all 4 serotypes, was used as a positive control Antibody-dependent enhancement assays on U937 cells.
Midgley, C. M., Flanagan, A., Tran, H. B., Dejnirattisai, W., Chawansuntati, K., Jumnainsong, A., ... & Screaton, G. R. (2012). Structural analysis of a dengue cross-reactive antibody complexed with envelope domain III reveals the molecular basis of cross-reactivity. The Journal of Immunology, 188(10), 4971-4979.
Specifications
- Immunogen
- Horse Equ c1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native DENV-4 EDIII
- Species Reactivity
- DENV-4
- Clone
- 2H12
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Dengue virus; DENV; dengue virus envelope protein domain III; DENV envelope protein domain III
- UniProt ID
- Q7TGC7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 2H12"
See other products for "ED3"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-301-S(P) | Recombinant Anti-ED3 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| PFBC-147 | Recombinant Mouse Anti-DENV-4 EDIII Antibody Fab Fragment (2D73) | ELISA | Fab |
| PFBC-148 | Recombinant Mouse Anti-DENV-4 EDIII Antibody Fab Fragment (3E31) | ELISA | Fab |
| PFBC-149 | Recombinant Mouse Anti-DENV-4 EDIII Antibody Fab Fragment (2H12) | ELISA | Fab |
| PFBC-150 | Recombinant Mouse Anti-DENV-3 EDIII Antibody Fab Fragment (2H12) | ELISA | Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-301 | Recombinant Human Anti-ED3 Antibody | WB, Neut, FuncS | IgG |
| PABC-147 | Recombinant Mouse Anti-DENV-4 EDIII Antibody (2D73) | ELISA | IgG |
| PABC-148 | Recombinant Mouse Anti-DENV-4 EDIII Antibody (3E31) | ELISA | IgG |
| PABC-150 | Recombinant Mouse Anti-DENV-3 EDIII Antibody (2H12) | ELISA | IgG |
| PABC-243 | Recombinant Mouse Anti-WNV ED3 Antibody (E16) | Neut | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-301-F(E) | Recombinant Human Anti-ED3 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| PSBC-147 | Recombinant Mouse Anti-DENV-4 EDIII Antibody scFv Fragment (2D73) | ELISA | scFv |
| PSBC-148 | Recombinant Mouse Anti-DENV-4 EDIII Antibody scFv Fragment (3E31) | ELISA | scFv |
| PSBC-149 | Recombinant Mouse Anti-DENV-4 EDIII Antibody scFv Fragment (2H12) | ELISA | scFv |
| PSBC-150 | Recombinant Mouse Anti-DENV-3 EDIII Antibody scFv Fragment (2H12) | ELISA | scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABC-149. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.