Recombinant Mouse Antibody (1A1D-2) is capable of binding to DENV E-DIII, expressed in Chinese Hamster Ovary cells (CHO). The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells.
Figure 1 Mouse Anti-DENV E-DIII Antibody (1A1D-2) in Neut.
Lateral ridge and A strand epitope mutant viruses escape neutralization by EDIII binding mouse MAbs. Neutralization assays were performed using parental and mutant viruses and a panel of mouse MAbs that bind to different regions of E protein. The graph displays the mean percentages of neutralization from two independent experiments for each MAb used at a concentration of 10 μg/ml.
Wahala, W. M., Huang, C., Butrapet, S., White, L. J., & de Silva, A. M. (2012). Recombinant dengue type 2 viruses with altered e protein domain III epitopes are efficiently neutralized by human immune sera. Journal of virology, 86(7), 4019-4023.
Figure 2 Mouse Anti-DENV E-DIII Antibody (1A1D-2) in SPR.
SPR sensorgrams for Fab of 1A1D-2 WT and mutants to DENV1-4. In the SPR experiments, recombinant DENV EDIII was covalently immobilized on the CM5 chip (Cytiva). A series of 1A1D-2 Fab (WT or mutants) in two-fold dilutions were flowed through the chip for two minutes and allowed to dissociated for three minutes. Colored lines represent experiment data at different analyte concentrations, and the black lines are fitting curve. Each experiment was independently repeated for three times. For DENV4, the highest concentration used in this experiment reached 3200 nM for recombinant EDIII proteins. The color keys were provided below the graphs.
Wei, H., Tan, J., Zhou, B., Guan, X., Zhong, Q., & Wang, J. (2022). Charged Residue Implantation Improves the Affinity of a Cross-Reactive Dengue Virus Antibody. International journal of molecular sciences, 23(8), 4197.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275 | Recombinant Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
MHH-275 | Recombinant Human Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
PABL-437 | Recombinant Mouse Anti-DENV EDIII Antibody (2H12) | ELISA, Neut | IgG |
MRO-1612CQ | Recombinant Human Anti-DENV E-DIII Antibody (DV10) | ELISA, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275-F(E) | Recombinant Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
MHH-275-F(E) | Recombinant Human Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
PFBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody Fab Fragment (1A1D-2) | WB, ELISA | IgG |
PFBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody Fab Fragment (2H12) | ELISA, Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275-S(P) | Recombinant Anti-DENV E-DIII Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
MHH-275-S(P) | Recombinant Human Anti-DENV E-DIII Antibody scFv Fragment | Neut, IF, FuncS | scFv |
PSBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody scFv Fragment (1A1D-2) | WB, ELISA | scFv |
PSBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody scFv Fragment (2H12) | ELISA, Neut | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-044 | Recombinant Mouse Anti-DENV Antibody (3E31) | WB, ELISA, FuncS, Neut, Inhibt | IgG |
PABX-045 | Recombinant Mouse Anti-DENV Antibody (4E11) | WB, ELISA, FuncS, IF | IgG |
PABX-054 | Recombinant Mouse Anti-DENV Antibody (E111) | WB, ELISA, FuncS, SPR, Neut | IgG |
PABX-044-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (3E31) | WB, ELISA, FuncS | scFv |
PABX-044-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (3E31) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-054-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (E111) | WB, ELISA, FuncS | scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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