Mouse Anti-DNA Recombinant Antibody (clone 64M-2); Fab Fragment (CAT#: PFBC-033)

Recombinant Mouse Antibody (64M-2) Fab Fragment is specific to DNA dT(6-4)T, expressed in Chinese Hamster Ovary cells (CHO).


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Figure 1 The binding of monoclonal antibodies to singlestranded DNA (50 ng/well) irradiated with various 254- nm UV doses.

Figure 1 The binding of monoclonal antibodies to singlestranded DNA (50 ng/well) irradiated with various 254- nm UV doses.

Monoclonal antibodies with 1/100 dilution were used. Each point shows the mean of three independent determinations.

Mori, T., Nakane, M., Hattori, T., Matsunaga, T., Ihara, M., & Nikaido, O. (1991). Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6‐4) photoproduct from the same mouse immunized with ultraviolet‐irradiated DNA. Photochemistry and photobiology, 54(2), 225-232.

Figure 2 The binding abilities of monoclonal antibodies to DNA damage induced by 254-nm UV irradiation (2 kJ/m²) and modification of binding abilities after subsequent 313-nm UV irradiation to the DNA.

Figure 2 The binding abilities of monoclonal antibodies to DNA damage induced by 254-nm UV irradiation (2 kJ/m²) and modification of binding abilities after subsequent 313-nm UV irradiation to the DNA.

Monoclonal antibodies with 1/100 or 1/1000 dilution were used. Data typical of two or three independent experiments are presented. The broken line shows the result of 64M-1 antibody.

Mori, T., Nakane, M., Hattori, T., Matsunaga, T., Ihara, M., & Nikaido, O. (1991). Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6‐4) photoproduct from the same mouse immunized with ultraviolet‐irradiated DNA. Photochemistry and photobiology, 54(2), 225-232.


Specifications

  • Immunogen
  • Deoxyribonucleic acid
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Clone
  • 64M-2
  • Applications
  • ELISA, WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody Fab fragment 64M-2 has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • DNA; Deoxyribonucleic acid; polynucleotides; double heliX

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Mouse Antibody

CAT Product Name Application Type
PABX-048 Recombinant Mouse Anti-DNA Antibody WB, ELISA, FuncS IgG
PABX-048-F (E) Recombinant Mouse Anti-DNA Antibody Fab Fragment WB, ELISA, FuncS Fab

Humanized Antibody

CAT Product Name Application Type
PABX-048-S (P) Recombinant Mouse Anti-DNA Antibody scFv Fragment WB, ELISA, FuncS scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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