Recombinant Mouse Anti-EBOV GP Antibody (14G7) (CAT#: PABW-038)

Recombinant Mouse Antibody (14G7) is capable of binding to EBOV GP, expressed in Chinese Hamster Ovary cells (CHO). Antibody 14G7 is protective against lethal Ebola virus challenge and recognizes a distinct linear epitope in the prominent mucin-like domain of the Ebola virus glycoprotein GP.


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Figure 1 VSR-marked organelle colocalized with AtPep12p-marked compartments in pea and tomato cells.

Figure 1 VSR-marked organelle colocalized with AtPep12p-marked compartments in pea and tomato cells.

Anti-VSR monoclonal antibodies (14G7 and 17F9) were compared to either anti-AtPep12p (AtSYP21) or anti-AtELP polyclonal antibodies via double labeling of confocal immunofluorescence in root-tip cells derived from pea (panels 1, 2, 4) and tomato (panels 3 and 4). The open arrows and arrowheads indicate examples of colocalization and separation of the two antibodies, respectively. n, nucleus. Bar = 10 mm.

Li, Y. B., Rogers, S. W., Tse, Y. C., Lo, S. W., Sun, S. S., Jauh, G. Y., & Jiang, L. (2002). BP-80 and homologs are concentrated on post-Golgi, probable lytic prevacuolar compartments. Plant and Cell Physiology, 43(7), 726-742.

Figure 2 Anti-BP-80 antibodies recognize the Arabidopsis BP-80 homolog VSR At-1.

Figure 2 Anti-BP-80 antibodies recognize the Arabidopsis BP-80 homolog VSR At-1.

The truncated form (missing its TMD/CT) of VSR At-1 , which is identical to AtELP, was expressed and secreted in insect cells. For Western blot detection, affinity-purified recombinant protein equivalent to 100 ml of culture media was loaded on to each lane, followed by SDS-PAGE and immunoblotting. Each identical strip was labeled by individual anti-BP-80 antibodies. The arrow indicates the position of the truncated AtBP-80. The individual antibody used in Western blot detection is indicated above each lane (1-5). The positions of molecular mass marker are indicated in kilodaltons on the left.

Li, Y. B., Rogers, S. W., Tse, Y. C., Lo, S. W., Sun, S. S., Jauh, G. Y., & Jiang, L. (2002). BP-80 and homologs are concentrated on post-Golgi, probable lytic prevacuolar compartments. Plant and Cell Physiology, 43(7), 726-742.


Specifications

  • Immunogen
  • EBOV spike glycoprotein
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native EBOV GP
  • Species Reactivity
  • EBOV
  • Clone
  • 14G7
  • Applications
  • ELISA, Neut

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The EBOV GP antibody has been reported in applications of IF, WB.

Target

  • Alternative Names
  • spike glycoprotein; GP

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Epitope-Specific Antibody

Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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