Products

Anti-EPCAM immunotoxin VB4-845 (scFv)-Bouganin (AGTO-G021O)

This immunotoxin was achieved by conjugating/fusing the Anti-EPCAM scFv to Bouganin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis and enzymatically damages ribosomes, ultimately leading to cell death. It was designed for treatment of Head and neck cancer.      

Specifications
Host
Mouse
Species Reactivity
Human
Clone
VB4-845
Molecule Class
scFv-Bouganin
Toxin Description
Bouganin is a type-I ribosome inactivating protein (RIP) that was recently purified from leaves of Bougainvillea spectabilis Willd. Like all type-I RIPs, bouganin is a RNA glycosidase with deadenylating activity. Bouganin was characterized as a single-chain protein with a molecular mass of 26 200 Da and its mature protein consists of 250 amino acids. Furthermore, it was found that bouganin is probably the least toxic RIP known based on protein inhibiting studies in cell lines. It was observed that the bouganin concentrations inhibiting protein synthesis of human cell lines were much higher than the concentrations required to inhibit protein synthesis in a rabbit reticulocyte lysate assay, a characteristic common to all type-I RIPs.
Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
Related Disease
Head and neck cancer
Target
Alternative Names
EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2
Entrez Gene ID
4072
UniProt ID
P16422

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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