Anti-ERBB2 immunotoxin 4D5 (scFv)-PE (CAT#: AGTO-G022E)
This immunotoxin was achieved by conjugating/fusing the Anti-ERBB2 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating SK-OV-3 tumor xenografts.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 4D5
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- SK-OV-3 tumor xenografts
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE48
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "Clone 4D5"
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Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-005 | Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) | FC, IP, ELISA, Neut, FuncS, IF, IHC | IgG1 - kappa |
| TAB-041CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-041CT-F(E)) | ELISA, WB | Humanized Fab |
| TAB-050CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (HuA21) | ELISA, WB | Humanized antibody |
| TAB-053CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-053CT-F(E)) | ELISA, Inhib, FC | Humanized Fab |
| TAB-054CT-F(E) | Humanized Anti-Human HER2/neu Recombinant Antibody Fab Fragment | ELISA, Inhibition, FC | Humanized antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr3) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr6) | SPR | Llama VHH |
| PNBL-069 | Recombinant Anti-HER2 VHH Single Domain Antibody (huMAb4D5-5) | ELISA | Llama VHH |
| HPAB-0201-FY | Recombinant llama Anti-ERBB2 Antibody (VHH6) | E | llama VHH |
| HPAB-0202-FY | Recombinant llama Anti-ERBB2 Antibody (VHH1) | E | llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-089 | Mouse Anti-ERBB2 Recombinant Antibody (clone A21); Fab Fragment | ELISA, WB, FC | Mouse Fab |
| HPAB-0733LY-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone AH06); Fab Fragment | ELISA | Human Fab |
| HPAB-0734LY-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone A058); Fab Fragment | ELISA | Human Fab |
| HPAB-0735LY-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone AH16); Fab Fragment | ELISA | Human Fab |
| HPAB-0736LY-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone A091); Fab Fragment | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (pertuzumab ) | WB | |
| TAB-036CT | Anti-Human HER2/neu Recombinant Antibody (6B2) | ELISA, WB | |
| TAB-037CT | Anti-Human HER2/neu Recombinant Antibody (7C2/7F3) | ELISA, WB | |
| TAB-270CQ | Mouse Anti-ERBB2 Recombinant Antibody (TAB-270CQ) | WB, Inhib | Mouse IgG |
| PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-042CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (CRX01) | WB | Human antibody |
| TAB-043CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-043CT-S(P)) | ELISA, Inhibion | Human scFv |
| TAB-044CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (MIL5) | WB, FC, IF, Funcs | Human antibody |
| TAB-055CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment | WB | Human antibody |
| TAB-056CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-056CT-S(P)) | WB | Human scFv |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-148LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-149LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0021-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-0021-YC) | ELISA, Neut, FC, FuncS | Human IgG |
| FAMAB-0098YC | Mouse Anti-ERBB2 Recombinant Antibody (clone E402) | Cyt | Mouse IgG1, κ |
| FAMAB-0099YC | Human Anti-ERBB2 Recombinant Antibody (clone CH402) | Cyt | Chimeric (mouse/human) IgG1 |
| HPAB-0091-FY | Human Anti-ERBB2 Recombinant Antibody (clone MIL12) | ELISA | Humanized IgG |
| HPAB-M0073-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-M0073-YC) | ELISA, FC | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1265WJ-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-1265WJ-S(P)) | ELISA | Humanized scFv |
| FAMAB-0172JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6H2); scFv Fragment | ELISA, FuncS | Human scFv |
| FAMAB-0173JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6H211); scFv Fragment | ELISA, FuncS | Human scFv |
| FAMAB-0359JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone FRc13); scFv Fragment | DB, ELISA, FuncS | Human scFv |
| PSBS-0070 | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (PSBS-0070) | ELISA, FuncS | Human scFv |
MHC Tetramer for Cancer
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