Anti-EV VP1 Recombinant Antibody (5-D8/1) (CAT#: MRO-1008LC)
This mouse monoclonal antibody is raised against VP3 of enteroviruses and reactive specifically with the structural VP3 of many kinds of enteroviruses. The IgG2a isotype antibody could be used to detect Enterovirus, Coxsackie A Virus, Coxsackie B Virus, Echovirus, Enterovirus 71, Poliovirus, and Rhinovirus in ELISA and IF applications.
Specific Inquiry
IHC
Figure 1. Lethal coxsackievirus myocarditis in a male neonate.Immunohistochemistry (IH) detects enteroviral VP1 in myocytes (clone5-D8/1, brown signals).
Strong background staining in the heart of a patient with systemic sclerosis and chronic inflammation using clone 5-D8/1 (brown signals).No viral RNA is detected by radioactive in situ hybridization in consec-utive tissue sections.
Ettischer-Schmid, N., Normann, A., Sauter, M., Kraft, L., Kalbacher, H., Kandolf, R., ... & Klingel, K. (2016). A new monoclonal antibody (Cox mAB 31A2) detects VP1 protein of coxsackievirus B3 with high sensitivity and specificity. Virchows Archiv, 469(5), 553-562.
WB
Figure 2. Cross-reactivity of clone 5-D8/1 with cellular proteins of HeLacells and human myocardium by Western blotting of HeLa cells infectedwith different enteroviruses and mock-infected cells.
Ettischer-Schmid, N., Normann, A., Sauter, M., Kraft, L., Kalbacher, H., Kandolf, R., ... & Klingel, K. (2016). A new monoclonal antibody (Cox mAB 31A2) detects VP1 protein of coxsackievirus B3 with high sensitivity and specificity. Virchows Archiv, 469(5), 553-562.
IF
Figure 3. Immunofluorescence staining of infected Vero cells to test theability of the serum from immunized mice to detect viral proteins.
CVB3-infected and fixed monolayers were incubated with different dilu-tions of sera (positive cells are red), nuclei were counterstained withDAPI. Positive control of infection was done by staining the infectedmonolayers with clone 5-D8/1
Ettischer-Schmid, N., Normann, A., Sauter, M., Kraft, L., Kalbacher, H., Kandolf, R., ... & Klingel, K. (2016). A new monoclonal antibody (Cox mAB 31A2) detects VP1 protein of coxsackievirus B3 with high sensitivity and specificity. Virchows Archiv, 469(5), 553-562.
IHC
Figure 1 Immunohistochemistry using the mAb 5-D8/1 in dilutions of 1 : 100 (A, F), 1 : 200 (B, D), and 1 : 800 (H, I) or the mitochondria mAb 113–1 in dilutions of 1 : 400 (E) and 1 : 800 (C, G, J–L) on sections of liver (A–C).
Hansson, S. F., Korsgren, S., Pontén, F., & Korsgren, O. (2013). Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross‐reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins. The Journal of pathology, 229(5), 719-728.
WB
Figure 2 Immunoblotting with mAb clone 5-D8/1 of islet tissue and exocrine tissue from the same individual.
Hansson, S. F., Korsgren, S., Pontén, F., & Korsgren, O. (2013). Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross‐reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins. The Journal of pathology, 229(5), 719-728.
Specifications
- Host Species
- Mouse
- Type
- Mouse antibody
- Specificity
- Enterovirus
- Clone
- 5-D8/1
- Applications
- ELISA, IHC, WB
- Related Disease
- Enterovirus infection
Applications
- Application Notes
- The EV VP1 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Western Blot.
Target
- Alternative Names
- Enterovirus; Enterovirus VP1; Coxsackie viruses; Echoviruses; Polioviruses; Rhinovirus 1A; Human rotavirus; Measles virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1008LC-S(P) | Anti-EV VP1 Recombinant Antibody scFv Fragment (5-D8/1) | ELISA | Mouse antibody |
| MRO-1008LC-F(E) | Anti-EV VP1 Recombinant Antibody Fab Fragment (MRO-1008LC-F(E)) | IF, ELISA | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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