Recombinant Mouse Anti-Electric eel AChE Antibody (mAb408) (CAT#: PABZ-040)

Figure 1 Inhibition of E. electricus AChE by inhibitory mAbs as a function of substrate concentration.

In these experiments, AChE was previously treated 24 h at 4°C with various concentrations of mAbs and the residual activity was measured as a function of substrate concentration (acetylthiocholine). R is the ratio of antibody-binding sites to AChE catalytic subunits. (A, B) Results obtained with Elec-403 and Elec-408, respectively. The results obtained with Elec-410 are similar to those of Elec-403 in that the maximal inhibition is observed only in the presence of a large excess of antibody (data not shown). (closed boxes) Control without antibody; (open boxes) control with non-inhibitory mAb Elec-106; (open triangles) R = 1; (closed triangles) R = 2; (closed circles) R = 20. (C) The results obtained with Elec-403 and Elec-410 when antibodies were used in large excess (R = 20). (closed circles) Elec- 403 ; (open triangles) Elec-410. The results obtained with Elec-408 cannot be represented using the same Y-axis scale, but it is clear from the data in B that substrate inhibition still occurs for R = 20.

Remy, M. H., Frobert, Y., & Grassi, J. (1995). Characterization of monoclonal antibodies that strongly inhibit Electrophorus electricus acetylcholinesterase. European journal of biochemistry, 231(3), 651-658.