Anti-FCGR1 immunotoxin H22 (IgG)-PE (CAT#: AGTO-G025E)
This immunotoxin was achieved by conjugating/fusing the Anti-FCGR1A monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to acute myeloid leukemia (AML) cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating acute myeloid leukemia.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/2005_G4_1_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/2005_G4_1_selected.jpg
Figure 2 IF staining of human cell line NB-4
(Immunofluorescent staining of human cell line NB-4 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/1714_A8_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line NB-4 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/1714_A8_1_blue_red_green.jpg
Figure 3 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/1712_D7_3_cr580511849a647_blue_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26910/1712_D7_3_cr580511849a647_blue_red_green.jpg
Figure 4 RNA cell line category: Cell line enriched (THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150337-FCGR1A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150337-FCGR1A
Figure 4 RNA cell line category: Cell line enriched (THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150337-FCGR1A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150337-FCGR1A
Specifications
- Host Species
- Human
- Species Reactivity
- Human
- Clone
- H22
- Molecule Class
- IgG-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- acute myeloid leukemia
Target
- Alternative Names
- FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); CD64; FCRI; CD64A; IGFR1; high affinity immunoglobulin gamma Fc receptor I; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; Fc fragment of IgG, high affinity Ia, receptor for (CD64); Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE51
- Gene ID
- 2209
- UniProt ID
- P12314
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-984 | Recombinant Mouse Anti-Human FCGR1A Antibody F(ab')2 Fragment, clone 10.1 | FC, WB | F(ab')2 |
| DrMAB-990 | Mouse Anti-FCGR1A Recombinant Antibody (clone 10.1); Fab Fragment | FC, WB | Mouse Fab |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G024E | Anti-FCGR1 immunotoxin H22 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G024R | Anti-FCGR1 immunotoxin H22 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G025R | Anti-FCGR1 immunotoxin H22 (IgG)-RTA | Cytotoxicity assay, Function study |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-032WM | Anti-Human FCGR1A Recombinant Antibody (611) | ELISA, FC, FuncS | Human Antibody |
| TAB-032WM-S(P) | Anti-Human FCGR1A Recombinant Antibody scFv Fragment (611) | ELISA, FC, FuncS | Human Antibody |
| TAB-032WM-F(E) | Anti-Human FCGR1A Recombinant Antibody Fab Fragment (611) | ELISA, FC, FuncS | Human Antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1269 | Hi-Affi™ Rabbit Anti-Fcgr1a Recombinant Antibody (clone DS1269AB) | ELISA | Rabbit IgG |
| MOR-1270 | Hi-Affi™ Rabbit Anti-FCGR1A Recombinant Antibody (clone DS1270AB) | FC, IP, WB | Rabbit IgG |
| MOR-1271 | Hi-Affi™ Rabbit Anti-Fcgr1a Recombinant Antibody (clone DS1271AB) | ELISA | Rabbit IgG |
| MOR-4066 | Hi-Affi™ Rabbit Anti-Fcgr1a Recombinant Antibody (clone SI133DS) | ELISA | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FN-010CQ | Mouse Anti-FCGR1A Recombinant Antibody (clone 10.1) | FC, Block, WB | Mouse IgG1 |
| VS3-XY680 | Mouse Anti-FCGR1A Recombinant Antibody, FITC (clone 4A10.1) | FC, IHC, ICC, IP, IF, WB | Mouse IgG1 |
| VS3-XY681 | Mouse Anti-FCGR1A Recombinant Antibody, PE (clone 4A10.1) | FC, IHC, ICC, IP, IF, WB | Mouse IgG1 |
| VS3-XY682 | Mouse Anti-FCGR1A Recombinant Antibody (clone 4A10.1) | FC, IHC, ICC, IP, IF, WB | Mouse IgG1 |
| VS4-WK173 | Mouse Anti-FCGR1A Recombinant Antibody (VS4-WK173) | ELISA, IF, FC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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