Human Anti-FAS Recombinant Antibody (clone HFE7A) (CAT#: PABL-094)

Recombinant Human Antibody (HFE7A) is capable of binding to Fas, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Fas mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Fas mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.


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Figure 1 Binding Assay to Human Fas-AIC2.

Figure 1 Binding Assay to Human Fas-AIC2.

The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.

Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.

Figure 2 Inhibition Assay against the Binding between m-HFE7A and Human Fas-AIC2.

Figure 2 Inhibition Assay against the Binding between m-HFE7A and Human Fas-AIC2.

The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.

Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.

Figure 3 Apoptosis Induction Assay against WR19L12a Cells.

Figure 3 Apoptosis Induction Assay against WR19L12a Cells.

The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.

Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.


Specifications

  • Immunogen
  • Human Fas cell surface death receptor
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human FAS
  • Species Reactivity
  • Human
  • Clone
  • HFE7A
  • Applications
  • ELISA, WB, IF, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Inhibition and Apoptosis. For details, refer to published data.

Target

  • Alternative Names
  • APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0201MZ Chicken Anti-FAS (ab1) Polyclonal IgY WB Chicken antibody
BRD-0202MZ Chicken Anti-FAS (ab2) Polyclonal IgY WB Chicken antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-767CQ Mouse Anti-FAS Recombinant Antibody (clone CBL933) Neut, FC, WB Mouse IgG1

Blocking Antibody

CAT Product Name Application Type
NEUT-768CQ Mouse Anti-FAS Recombinant Antibody (clone ZB4) Block, FC, IHC, WB Mouse IgG1

Rabbit Monoclonal Antibody

Agonistic Antibody

CAT Product Name Application Type
MOB-103LC Mouse Anti-FAS Recombinant Antibody (clone CH11) FC, ICC, WB

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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