Human Anti-Ferritin Recombinant Antibody (clone F11) (CAT#: PABW-044)
Recombinant Human Antibody (F11) is capable of binding to Ferritin, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Ferritin mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Ferritin mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. F11-reactive epitope on human spleen ferritin differs somewhat from that on rL-ferritin and,that these differences do not dramatically change the binding affinity of the parent full-length antibody.
Specific Inquiry
Figure 1 HNoncompetitive ELISA of ferritin binding by antibody F11 (b) and its isolated VLLdomain (a); K a = 6.5⋅10 8 (b) and 1.2⋅10 8 M-1 (a), respectively.
Immobilized VLLdomain or antibody F11 were interacted with increasing amounts of ferritin.
Dubnovitsky, A. P., Kravchuk, Z. I., Chumanevich, A. A., Cozzi, A., Arosio, P., & Martsev, S. P. (2000). Expression, refolding, and ferritin-binding activity of the isolated VL-domain of monoclonal antibody F11. BIOCHEMISTRY C/C OF BIOKHIMIIA, 65(9), 1011-1018.
Specifications
- Immunogen
- Human Ferritin
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Ferritin
- Species Reactivity
- Human
- Clone
- F11
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Ferritin antibody has been reported in applications of Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Ferritin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone F11"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-355 | Recombinant Anti-Ferritin Antibody | ELISA, WB, FuncS | IgG |
| MHH-355 | Recombinant Human Anti-Ferritin Antibody | WB, FC, FuncS | IgG |
| MOB-0107MZ | Recombinant Mouse Anti-Ferritin Antibody (clone A091-12711) | ELISA | Mouse antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-355-F(E) | Recombinant Anti-Ferritin Antibody Fab Fragment | IF, ELISA, FuncS | Fab |
| MHH-355-F(E) | Recombinant Human Anti-Ferritin Antibody Fab Fragment | WB, IP, FuncS | Fab |
| PFBW-044 | Human Anti-Ferritin Recombinant Antibody (clone F11); Fab Fragment | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-355-S(P) | Recombinant Anti-Ferritin Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-355-S(P) | Recombinant Human Anti-Ferritin Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| PSBW-044 | Human Anti-Ferritin Recombinant Antibody (clone F11); scFv Fragment | ELISA | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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