Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3) (CAT#: PABZ-046)
Recombinant Human Antibody (4M5. 3) is capable of binding to Fluorescein, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Fluorescein mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Fluorescein mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Labeling of fluorescein-conjugated yeast with purified 4m5.3 IgG followed by an anti-human PE-conjugated antibody and analysis by flow cytometry.
A type-matched, non-specific, human IgG1 k polyclonal antibody (Hu IgG1 polyclonal) and cells labeled with biotin (PEG-biotin) instead of fluorescein were used as negative controls. Pre-incubation of the 4m5.3 IgG with 10 mM fluorescein was also done to demonstrate specificity.
Rakestraw, J. A., Sazinsky, S. L., Piatesi, A., Antipov, E., & Wittrup, K. D. (2009). Directed evolution of a secretory leader for the improved expression of heterologous proteins and full‐length antibodies in Saccharomyces cerevisiae. Biotechnology and bioengineering, 103(6), 1192-1201.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Clone
- 4M5.3
- Applications
- FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 4M5.3 has been reported in application of Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Flow Cytometry: The reported concentration of 4M5.3 for flow cytometry was 10 μg/mL.
Target
- Alternative Names
- Fluorescein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-3 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | WB, FC | Human IgG |
| DrMAB-13 | Mouse Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Mouse IgG |
| DrMAB-23 | Rat Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Rat IgG |
| PABL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1) | ELISA, WB, FuncS | Mouse IgG |
| FAMAB-0113YC | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-s-3) | ELISA | Chicken IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-6 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20); Fab Fragment | WB, FC | Human Fab |
| PFBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); Fab Fragment | FC | Human Fab |
| PFBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); Fab Fragment | WB, FuncS | Human Fab |
| PFBL-472 | Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20); Fab Fragment | Neut | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| PSBL-472 | Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20); scFv Fragment | Neut | Mouse scFv |
| FAMAB-0113-YC-S(P) | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-s-3); scFv Fragment | ELISA | Chicken scFv |
| FAMAB-0114-YC-S(P) | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-F-8); scFv Fragment | ELISA | Chicken scFv |
| FAMAB-0141CQ-S(P) | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3); scFv Fragment | ELISA | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037 | Recombinant Mouse Anti-fluorescein Antibody (4-4-20) | WB, ELISA, FuncS, PK | IgG |
| PABX-037-F (E) | Recombinant Mouse Anti-fluorescein Antibody Fab Fragment (4-4-20) | WB, ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037-S (P) | Recombinant Mouse Anti-fluorescein Antibody scFv Fragment (4-4-20) | WB, ELISA, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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