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Recombinant Anti-human G3BP Intrabody [+36 GFP] (CAT#: IAB-B016(G))

Recombinant anti-G3BP intrabody in fusion with +36GFP works within cells to bind intracellur antigen human G3BP, expressed in 293T cells. This inhibition is attributed to the blocking of the endogenous G3BP protein.


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  • Gene Expression
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/if_selected.jpg
Figure 4 IF staining of human cell line A-431
Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/27_D12_1_blue_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/26_D12_1_blue_red_green.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/ihc_selected.jpg
Figure 3 IHC staining of human duodenum
Figure 3 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37068/ihc_selected.jpg
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_B_7_5.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_3.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_8_5.jpg
Figure 9 Testis
Figure 9 Testis
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_4_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145907-G3BP1

Specifications

  • Immunogen
  • human GTPase activating protein (SH3 domain) binding protein 1
  • Host Species
  • llama
  • Type
  • vhh-(+36GFP)
  • Cell Penetrating Peptide
  • +36 GFP
    GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
  • Species Reactivity
  • human
  • Applications
  • Used for immunoassay techniques such as: Western blot; Coimmunopricipation; Functional studies

Product Property

  • Purity
  • Purity>95% by SDS-PAGE
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • G3BP1; GTPase activating protein (SH3 domain) binding protein 1; G3BP; HDH-VIII; ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuc

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "G3BP"

Intrabody

CAT Product Name Application Type
IAB-B016(A) Recombinant Anti-human G3BP Intrabody [(D-Arg)9] IF, FC, WB, FuncS vhh-(D-Arg)9
IAB-B016(T) Recombinant Anti-human G3BP Intrabody [Tat] IF, ELISA, FuncS vhh-Tat

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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