Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • GLI3
  • Recombinant Anti-human GLI3 Intrabody [(D-Arg)9]

Recombinant Anti-human GLI3 Intrabody [(D-Arg)9] (CAT#: IAB-B069(A))

Recombinant anti-GLI3 intrabody linked to (D-Arg)9 works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.


See more Engineered Antibody products for GLI3
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/61_A4_2_selected.jpg
Figure 3 IF staining of human cell line HeLa
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/1975_H4_2_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/59_A4_1_red_green.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_B_7_5.jpg
Figure 6 Testis
Figure 6 Testis
(Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106571-GLI3

Specifications

  • Immunogen
  • human GLI family zinc finger 3
  • Host Species
  • llama
  • Type
  • vhh-(D-Arg)9
  • Cell Penetrating Peptide
  • (D-Arg)9
    It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
  • Species Reactivity
  • human
  • Applications
  • Formats of immunological tests: Immunofluorescence; Flow Cytometry; Western blot; Functional studies

Product Property

  • Purity
  • >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
  • Storage
  • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

Target

  • Alternative Names
  • GLI3; GLI family zinc finger 3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; transcriptional activator GLI3; oncogene GLI3; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "GLI3"

Recombinant Antibody

CAT Product Name Application Type
MOB-396 Recombinant Anti-human GLI3 Antibody WB, IP, FuncS IgG
MHH-396 Recombinant Human Anti-human GLI3 Antibody ELISA, WB, Neut, FuncS IgG
MOB-0773CT Recombinant Mouse anti-Human GLI3 Monoclonal antibody (EML508) WB
MRO-0659-CN Rabbit Anti-GLI3 Recombinant Antibody (clone JG83-39) WB, IF Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
MOB-396-F(E) Recombinant Anti-human GLI3 Antibody Fab Fragment FC, IP, FuncS Fab
MHH-396-F(E) Recombinant Human Anti-human GLI3 Antibody Fab Fragment IF, Neut, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-396-S(P) Recombinant Anti-human GLI3 Antibody scFv Fragment ELISA, WB, FuncS scFv
MHH-396-S(P) Recombinant Human Anti-human GLI3 Antibody scFv Fragment FC, RIA, Biosensors, FuncS scFv

Intrabody

CAT Product Name Application Type
IAB-B069(G) Recombinant Anti-human GLI3 Intrabody [+36 GFP] ICC, Neut, FuncS vhh-(+36GFP)
IAB-B069(T) Recombinant Anti-human GLI3 Intrabody [Tat] ELISA, WB, ICC, FuncS vhh-Tat

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1452 Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB) ICC, IF, WB Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for IAB-B069(A). Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart
    Inquiry

    Go to compare