Products

Anti-GLI3 Intrabody [Tat] (IAB-B069(T))

Recombinant anti-GLI3 intrabody in fusion with HIV Tat works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.      

Specifications
Immunogen
human GLI family zinc finger 3
Host
llama
Type
vhh-Tat
Cell Penetrating Peptide
TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
Species Reactivity
human
Applications
Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies
Target
Alternative Names
GLI3; GLI family zinc finger 3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A
Entrez Gene ID
2737
UniProt ID
P10071

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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