Mouse Anti-GPC3 Recombinant Antibody (clone GC33) (CAT#: HPAB-0561-CN)
An anti-glypican 3 antibody GC33 which has strong GPC3 binding activity. GC33 also exhibits particularly strong CDC activity.
Specific Inquiry
WB
Figure 1 Anti-GPC3 Antibody (HPAB-0561-CN) in WB
Western blot analysis was performed with 0.05 µg (lane 1) and 0/1 µg (lane 2) recombinant human GPC3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with GPC3 Antibody (HPAB-0561-CN) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
ELISA
Figure 2 Anti-GPC3 Antibody (HPAB-0561-CN) in ELISA
ELISA analysis of HPAB-0561-CN was performed by coating with recombinant human GPC3 antigen. Then blocked with BSA, and incubated with GPC3 Antibody (HPAB-0561-CN) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- A fusion protein containing a peptide from 524 Ala to 563 Lys of GPC3 and GST
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- GC33
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Glypican 3; Intestinal Protein OCI-5; Glypican Proteoglycan 3; GTR2-2; MXR7; Heparan Sulphate Proteoglycan; Secreted Glypican-3;
OCI-5; SGBS1; DGSX; SGBS; SDYS; OCI5; SGB
- Gene ID
- 2719
- UniProt ID
- P51654
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone GC33"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| TAB-416CQ | Anti-Human GPC3 Therapeutic Single Domain Antibody (HN3) | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H14 | Anti-Human GPC3 Recombinant Antibody (Codrituzumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG1 - kappa |
| TAB-160CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-160CT-F(E)) | FC | Humanized Fab |
| TAB-161CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-161CT-F(E)) | FC | Humanized Fab |
| TAB-162CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-162CT-F(E)) | FC | Humanized Fab |
| TAB-163CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-163CT-F(E)) | FC | Humanized Fab |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G035E | Anti-Gpc3 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-157CT | Human Anti-GPC3 Recombinant Antibody (TAB-157CT) | ELISA, FC, IHC, WB | Chimeric (mouse/human) IgG1 |
| TAB-158CT | Human Anti-GPC3 Recombinant Antibody (TAB-158CT) | ELISA, FC, IHC, WB | Chimeric (mouse/human) IgG1 |
| TAB-157CT-S(P) | Mouse Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-157CT-S(P)) | ELISA, FC, IHC, WB | Mouse scFv |
| TAB-157CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-157CT-F(E)) | ELISA, FC, IHC, WB | Chimeric (mouse/human) Fab |
| TAB-158CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-158CT-F(E)) | ELISA, FC, IHC, WB | Chimeric (mouse/human) Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-167CT | Human Anti-GPC3 Recombinant Antibody (TAB-167CT) | ELISA, FC | Human IgG |
| TAB-167CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-167CT-S(P)) | ELISA, FC | Human scFv |
| TAB-152CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-152CT-F(E)) | ELISA, FC, IF, WB | Human Fab |
| TAB-154CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-154CT-F(E)) | ELISA, FC, IF, WB | Human Fab |
| TAB-156CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-156CT-F(E)) | ELISA, FC, IF, WB | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-263CQ | Anti-Human GPC3 Recombinant Antibody (YP7) | ELISA, Inhib, IHC | |
| TAB-263CQ-S(P) | Anti-Human GPC3 Recombinant Antibody scFv Fragment (YP7) | ELISA, WB, FC, IHC | |
| TAB-263CQ-F(E) | Anti-Human GPC3 Recombinant Antibody Fab Fragment (YP7) | ELISA, WB, FC, IHC |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC033 | PE-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-LC034 | APC-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-LC035 | BV421-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-YF297 | A*02:01/Human GPC3 (FVGEFFTDV) MHC Monomer | MHC Multimer | |
| MHC-YF468 | H-2Kd/Human GPC3 (EYILSLEEL) MHC Pentamer | FCM |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1486 | Hi-Affi™ Rabbit Anti-GPC3 Recombinant Antibody (clone DS1486AB) | IHC-P | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0553-CN-F(E) | Human Anti-GPC3 Recombinant Antibody (clone GES1); Fab Fragment | ELISA, FC, IF, IP | Human Fab |
| HPAB-0554-CN-F(E) | Human Anti-GPC3 Recombinant Antibody (clone huG5S1); Fab Fragment | ELISA, FC, IF, IP | Humanized Fab |
| HPAB-0556-CN-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0556-CN-F(E)) | ELISA, WB | Human Fab |
| HPAB-0558-CN-F(E) | Mouse Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0558-CN-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0560-CN-F(E) | Mouse Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0560-CN-F(E)) | ELISA, WB | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1697LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone G5S8); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-1698LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone GES2); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-1699LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone GES6); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-1700LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone GES8); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-2315LY-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (HPAB-2315LY-S(P)) | ELISA | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H14 | Afuco™ Anti-GPC3 ADCC Recombinant Antibody (Codrituzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2313LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2313LY) | ELISA | Human IgG |
| HPAB-2314LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2314LY) | ELISA | Human IgG |
| HPAB-2315LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2315LY) | ELISA | Human IgG |
| HPAB-M0097-YC | Human Anti-GPC3 Recombinant Antibody (clone PA21) | ELISA, WB | Human IgG |
| VS-0723-WK20 | Human Anti-GPC3 Recombinant Antibody (VS-0723-WK20) | ELISA | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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