Recombinant Human Anti-H1N1 HA Antibody (CH67) (CAT#: PABL-483)

Recombinant Human Antibody (CH67) is capable of binding to H1N1 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H1N1 HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-H1N1 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.


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IF

Figure 1 Representative confocal fluorescence microscopy images of HEp-2 cell staining.

Figure 1 Representative confocal fluorescence microscopy images of HEp-2 cell staining.

No primary antibody control-no prim and anti-HIV-1 MPER mAb 2F5 were used as controls.

Bajic, G., van der Poel, C. E., Kuraoka, M., Schmidt, A. G., Carroll, M. C., Kelsoe, G., & Harrison, S. C. (2019). Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies. Scientific reports, 9(1), 1-9.

FC

Figure 2 Protein microarray binding of influenza HA bnAbs.

Figure 2 Protein microarray binding of influenza HA bnAbs.

Representative ProtoArray summary for protein arrays blotted with CH67. Axis values are relative fluorescence signal intensity (MFI) in the 151 K array (y axis) or test mAb array (x axis). Each dot represents the average of duplicate array proteins.

Bajic, G., van der Poel, C. E., Kuraoka, M., Schmidt, A. G., Carroll, M. C., Kelsoe, G., & Harrison, S. C. (2019). Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies. Scientific reports, 9(1), 1-9.


Specifications

  • Immunogen
  • H1N1 IAV haemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H1N1 HA
  • Species Reactivity
  • H1N1 IAV
  • Clone
  • CH67
  • Applications
  • IF, FC

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • This antibody has been reported for use in Immunofluorescence and Flow Cytometry.

Target

  • Alternative Names
  • HA; haemagglutinin; HA1; HA2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Human Antibody

Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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